Correction to: Reactivation of TAp73 tumor suppressor by protoporphyrin IX, a metabolite of aminolevulinic acid, induces
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Cell Division Open Access
CORRECTION
Correction to: Reactivation of TAp73 tumor suppressor by protoporphyrin IX, a metabolite of aminolevulinic acid, induces apoptosis in TP53‑deficient cancer cells Alicja Sznarkowska1, Anna Kostecka1, Anna Kawiak1, Pilar Acedo2, Mattia Lion3,4, Alberto Inga3 and Joanna Zawacka‑Pankau2*
Correction to: Cell Div (2018) 13:10 https://doi.org/10.1186/s13008-018-0043-3
The authors note a correction to the article [1]. Figure 1d of the original article has an error. The pcDNA3/Ctrl and the pcDNA3-TAp73a/DMSO wells are duplicated. This article presents the corrected version of Fig. 1.
*Correspondence: [email protected] 2 Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Biomedicum, Solnavägen 9, 171 65 Stockholm, Sweden Full list of author information is available at the end of the article © The Author(s) 2019. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/ publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
Sznarkowska et al. Cell Div
(2019) 14:10
a
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P1 (TA-promoter) 1
2
γ ζ δ β
P2 (ΔN-promoter)
3
3B
4
5
6
7
8
9
10 11 12 13 14
α ΔN’ Δ2
3
Δ2/3
TAp73α
TA
DBD
OD
b
CT
c HCT 116 p53-/-
PpIX (μg/ml) DMSO
1
5
10
TAp73α actin
120 100 % of living cells
HCT 116 p53-/H1299 Saos2
pcDNA3 pcDNA3-TAp73α
80 60 40 20
DMSO
d
1
5
10
PpIX (μg/ml)
e HCT 116 p53-/Ctrl
DMSO
HCT 116 p53-/-
5
10 PpIX (μg/ml)
pcDNA3
pcDNA3-TAp73α
g 2.5 PpIX 20 CDDP
6
0
4
PARP-1
Cleaved 85 kDa
2
actin
DMSO
H2O2
1
5
24
48 48 [h]
CDDP
NHDF, 48h
RITA
8
PpIX
HCT 116 p53-/-
10
Ctrl
Tail moment (arbitrary units)
f
PARP-1
Cleaved 85 kDa
actin
PpIX (μg/ml)
Fig. 1 PpIX inhibits proliferation of cancer cells lacking p53. a Schematic representation of the splice variants in TP73 gene (upper panel) and domain structure of p73 (lower panel). TA transactivation domain, DBD DNA binding domain, OD oligomerization domain, CTC terminus. b PpIX induces dose-dependent growth inhibition in a long-term proliferation assay. c Ectopic expression of TAp73α sensitizes cells to PpIX after 24 h as demonstrated by WST-1 proliferation assay. Inserted blot represents the level of expression of TAp73α. Please note that the blot has been cropped. Dotted line represents where the blot has been cut. The uncropped full length version is presented in Additional file 3: Figure S3a. d TAp73α overexpression sensitizes H1299 to PpIX-induced inhibition of proliferation. e, f PpIX does not induce DNA damage in cancer cells at the effective therapeutic concentrations. g 2
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