Detection of clinically important non tuberculous mycobacteria (NTM) from pulmonary samples through one-step multiplex P
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RESEARCH ARTICLE
Open Access
Detection of clinically important non tuberculous mycobacteria (NTM) from pulmonary samples through one-step multiplex PCR assay Kamal Singh, Richa Kumari, Rajneesh Tripathi, Smita Gupta and Shampa Anupurba*
Abstract Background: The burden of non-tuberculous mycobacterial (NTM) disease is increasing worldwide but still its diagnosis is delayed and it is mistaken as multidrug-resistant tuberculosis (MDR-TB).The present study was performed to develop a multiplex PCR assay for detection and identification of clinically most common NTM to the species level from pulmonary samples. Results: Out of 50 isolates, 26 were identified as Mycobacterium kansasii (MK), 20 were identified as Mycobacterium abscessus (MA) and 4 were identified as Mycobacterium avium complex (MAC) through multiplex PCR and further confirmed by sequencing. Conclusion: Our study showed that multiplex PCR assay is a simple, convenient, and reliable technique for detection and differential identification of major NTM species. Keywords: NTM, Multiplex PCR, MTBC, MOTT, Mycobacterium avium complex, Mycobacterium abscessus and Mycobacterium kansasii
Background Tuberculosis (TB), caused by Mycobacterium tuberculosis complex (MTBC), persists as the principal killer disease worldwide, notably in the developing countries and has been a major public health problem in spite of considerable progress in diagnosis and treatment [1]. The genus Mycobacterium comprises several species that are divided into three groups, the MTBC, Mycobacterium leprae and atypical or non-tuberculous mycobacteria (NTM) [2]. NTM, also known as environmental mycobacteria or mycobacteria other than tuberculosis (MOTT), are mycobacteria which are generally free-living organisms and found ubiquitously in the environment [3]. There has * Correspondence: [email protected] Department of Microbiology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India
been approximately 200 NTM species identified to-date [4]. They can cause a wide range of infections, with pulmonary infections being the most frequent (65–90%) [5]. Nowadays NTM have become important human pathogens as the incidence and prevalence of disease caused by them continue to increase worldwide [6]. The disease causing agents among NTM differ geographically, but the most common species that are frequently isolated from patients with NTM infection are Mycobacterium avium complex (MAC) (Mycobacterium avium, Mycobacterium intracellulare and Mycobacterium chimaera), Mycobacterium abscessus complex (Mycobacterium abscessus subspecies bolletii, subspecies massiliense and Mycobacterium chelonae) and Mycobacterium kansasii [7, 8]. The identification and differentiation of NTM from MTBC is of important diagnostic value as the
© The Author(s). 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit t
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