Development of the method for quantification of amino acid adsorbed on nanoparticle surface
Bio-functionalization of nanoparticles with amino acids increases their biocompatibility and make them more efficient in delivery systems, especially in drug delivery and gene therapy. This can lead to development of new detection methods, in the field of
- PDF / 172,604 Bytes
- 5 Pages / 595.276 x 790.866 pts Page_size
- 84 Downloads / 209 Views
national Burch University, Francuske revolucije bb, Sarajevo, Bosnia and Herzegovina 2 Graz University of Technology, Rechbauerstraße 12, 8010, Graz Austria 3 Faculty of Agriculture and Food sciences, Zmaja od Bosne 8, Sarajevo, Bosnia and Herzegovina [email protected] [email protected] [email protected] [email protected] [email protected] [email protected]
Abstract. Bio-functionalization of nanoparticles with amino acids increases their biocompatibility and make them more efficient in delivery systems, especially in drug delivery and gene therapy. This can lead to development of new detection methods, in the field of clinical diagnostics, biosensors and DNA receptors. Alongside, understanding of the mechanism of amino acid adsorption on nanoparticle surface contributes to the evaluation of complex interaction between nanoparticles and proteins. Thus, development of methods for amino acid quantitation and characterization is very important. Still, the number of methods is very limited. We report a strategy for the quantification of cysteine adsorbed on gold, silver and silica nanoparticles by modified ninhydrin method. In order to obtain the most suitable ratio for amino acid adsorption, optimized parameters were: amino acid concentration, ratio of amino acids to nanoparticles, and nanoparticle concentration. Values of absorbance were measured by UV-Vis spectrophotometry and used for calculating the mass of adsorbed amino acid. The size of nanoparticles lacked an effect on amino acids whereas the ratio of amino acid to nanoparticles was revealed as a critical parameter. The method suggests 9:1 ratio as the most suitable for amino acid-nanoparticle interaction in case of both, gold and silica nanoparticles. Keywords: Gold nanoparticles, Silica nanoparticles, Amino acids, Ninhydrin method, Spectrophotometry Conflicts of interest: The authors declare that they have no conflict of interest regarding the publication of this paper.
© Springer Nature Singapore Pte Ltd. 2017 A. Badnjevic (ed.), CMBEBIH 2017, IFMBE Proceedings 62, DOI: 10.1007/978-981-10-4166-2_27
1
Introduction
1.1
Nanoparticles
Nanoparticles (NPs) are particles with at least one dimension in range from 1-100 nm [1]. The NPs exhibit unique physical properties such as particle aggregation, photoemission, electrical and heat conductivities; chemical properties such as catalytic activity; quantum properties and ability to tailor its surface that can be suitably manipulated for applications of interest [2]. The most important property of nanoparticles is a relatively large functional surface which makes NPs properties different from bulk material. The NPs surface is a medium where interactions between NPs and biomolecules occur, and a place where binding and adsorption of different compounds such as drugs, probes, peptides and proteins start [3]. The above mentioned characteristics, hitched up scientists and researchers all over the globe to experiment with nanoparticles, particularly biofunctio
Data Loading...
