Development of the Micro-Silk Through the Breeding of Transgenic Silkworm
The purpose of this research is to develop the micro-silk through the breeding of the transgenic silkworm. First of all, to construct transformation vector system of silkworm, we did cloning the sericin promoter in silkworm and sorting the selection marke
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Abstract The purpose of this research is to develop the micro-silk through the breeding of the transgenic silkworm. First of all, to construct transformation vector system of silkworm, we did cloning the sericin promoter in silkworm and sorting the selection marker gene (in order that sorting the transformation silkworm) and then cloning again. Second, we analyzed the fibroin gene of silkworm and IGF-1 gene of human After cloning. Then, we made and analyzed transformants of silkworm which gene expression as IGF-1 of human and the fibroin of silkworm. We inserted the protein expression vector which IGF-1 of human and the fibroin of silkworm into silkworm eggs using the microinjection. Using PCR and Southern blot methods, we analyzed expression vector in DNA level. In addition, we analyzed it using the RT-PCR methods in order to confirm gene expression in RNA level. The protein expression was analyzed in protein level through the western blot and ELISA methods. Through transformation on two separate occasions on spring and summer, we determined growth and ecology between Uzbekistan silkworm and Korean transformation silkworm. Lastly, we compared fabric of Uzbekistan silk, Chinese silk and Japanese 6A which we developed. Also we analyzed sericin content, strength and elongation and thickness of transformation silk single yarn. Keywords Transgenic silkworm
Sericin IGF-1 RT-PCR method
K.-B. Kim (&) Department of Textile Design, Gyeongnam National University of Science and Technology, 33, Dongjin-ro, Jinju, Korea e-mail: [email protected] M.-J. Kim Kyoto Institute of Technology, 1, Matsugasaki, Sakyo-ku, Kyoto, Japan e-mail: [email protected] K.-M. Choi BJ Silk, 17, Worasan-ro 996beon-gil Munsan-eup, Jinju, Korea e-mail: [email protected] © Springer International Publishing Switzerland 2017 W. Chung and C.S. Shin (eds.), Advances in Affective and Pleasurable Design, Advances in Intelligent Systems and Computing 483, DOI 10.1007/978-3-319-41661-8_4
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1 Introduction In the ongoing market, competitiveness of a certain product is one of the most important quality required to survive in the global market. So obtaining better quality and high yield of silk fiber from a single cocoon has been a major interest among people in this field for many decades [1–3]. In order to improve the quality of the silkworm cocoons we applied our biological technology which resulted in getting high quality micro-silk which width is less than 1 den [4, 5]. This micro-silk was obtained from a transgenic silkworm prepared by in vivo electroporation of expression vector into the silkworm [6]. At the same time, we were able to obtain high yield of IGF-1 (Insulin-like Growth Factor-1) from this transgenic silkworm in the sericin part also made from an expression vector including human IGF-1 clone. IGF-1 is a hormone similar in molecular structure to insulin. It plays an important role in childhood growth and continues to have anabolic effects in adults. A synthetic analog of IGF-1, mecasermin, is used for the treatment o
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