Effect of Nano-zirconia on Microstructure and Biological Behavior of Hydroxyapatite-Based Bone Scaffolds
- PDF / 3,263,904 Bytes
- 9 Pages / 593.972 x 792 pts Page_size
- 14 Downloads / 191 Views
JMEPEG https://doi.org/10.1007/s11665-020-04927-2
Effect of Nano-zirconia on Microstructure and Biological Behavior of Hydroxyapatite-Based Bone Scaffolds H. Maleki-Ghaleh, J. Khalil-Allafi, P. Keikhosravani, M.R. Etminanfar, and Y. Behnamian (Submitted August 12, 2019; in revised form June 2, 2020) In this research study, the effect of zirconia nanoparticles on the biological properties of the hydroxyapatite (HA) scaffolds was evaluated. The zirconia nanoparticles with various weight percentages of 5, 10, and 20 were mixed with hydroxyapatite powder using a ball-milling process. Then, they were cold pressed and heat-treated at 1150 °C. Scanning electron microscope and x-ray diffraction analysis were used to evaluate the morphology and phase analysis of the samples, respectively. The results of the microstructure and phase analysis revealed that some zirconia nanoparticles reacted with the HA during the sintering process, which besides the formation of the tertiary calcium phosphate and calcium zirconium phases, it resulted in creating some microporosities in the scaffold. The biological behavior of the samples was investigated by osteoblast-like cells. The results of the biological assessment demonstrated that the presence of the zirconia nanoparticles in the HA scaffold improved the biological behavior (cell attachment and cell proliferation). The HA specimen composed with 10 wt.% zirconia nanoparticles showed the highest bioactivity. In addition, the compressive strength of the HA sample composed of 10 wt.% zirconia nanoparticles was improved by 30%. Keywords
bone scaffold, biological behavior, hydroxyapatite, nano zirconia
composite,
1. Introduction Over the last few years, a great deal of attention has been given to developing the bone replacement materials (such as bone scaffolds), used to fill the areas of the damaged bone tissue (Ref 1-4). High bioactivity and mechanical strength are the two crucial features of the bone scaffolds. Among the bioactive biocosmetics, HA and tricalcium phosphate (TCP) have been used as the most important family of the calcium phosphates since the 1970s as the bone scaffolds in the body (Ref 5). TCP has a similar stoichiometry to the amorphous primary bone phase, and HA has a similar stoichiometry to the bone mineral (Ref 6). By placing the calcium phosphate ceramic scaffold near the healthy bone, the bone cells will be absorbed, growing on the surface of the implant (Ref 7). The bone cell will be mineralized after they grow on the surface of the scaffolds, making new bone tissues at the interface between
H. Maleki-Ghaleh, Research Center for Pharmaceutical Nanotechnology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran; Faculty of Materials Science and Engineering, K. N. Toosi University of Technology, Tehran, Iran; and Research Center for Advanced Materials, Faculty of Materials Engineering, Sahand University of Technology, P.O. Box 51335-11996, Tabriz, Iran; J. Khalil-Allafi and M.R. Etminanfar, Research Center for Advanced Materials, Faculty of Mat
Data Loading...
