Effects on steroid hormones secretion resulting from the acute stimulation of sectioning the superior ovarian nerve to p
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RESEARCH
Open Access
Effects on steroid hormones secretion resulting from the acute stimulation of sectioning the superior ovarian nerve to pre-pubertal rats Leticia Morales-Ledesma1*, Elizabeth Vieyra1, Deyra A Ramírez1, Angélica Trujillo3, Roberto Chavira2, Mario Cárdenas2 and Roberto Domínguez1
Abstract In the adult rat, neural signals arriving to the ovary via the superior ovarian nerve (SON) modulate progesterone (P4), testosterone (T) and estradiol (E2) secretion. The aims of the present study were to analyze if the SON in the pre-pubertal rat also modulates ovarian hormone secretion and the release of follicle stimulating hormone (FSH) and luteinizing (LH) hormone. P4, T, E2, FSH and LH serum levels were measured 30 or 60 minutes after sectioning the SON of pre-pubertal female rats. Our results indicate that the effects on hormone levels resulting from unilaterally or bilaterally sectioning the SON depends on the analyzed hormone, and the time lapse between surgery and autopsy, and that the treatment yielded asymmetric results. The results also suggest that in the pre-pubertal rat the neural signals arriving to the ovaries via the SON regulate the enzymes participating in P4, T and E2 synthesis in a non-parallel way, indicating that the mechanisms regulating the synthesis of each hormone are not regulated by the same signals. Also, that the changes in the steroids hormones are not explained exclusively by the modifications in gonadotropins secretion. The observed differences in hormone levels between rats sacrificed 30 and 60 min after surgery reflect the onset of the compensatory systems regulating hormones secretion. Keywords: Superior ovarian nerve, Steroid hormones, Gonadotropins, Pre-pubertal rat
Background In the rat, the ovary receives sympathetic innervation via two neural pathways: the ovarian plexus nerve (OPN), which accompanies the ovarian artery, and the superior ovarian nerve (SON), which travels along the suspensory ligament [1,2]. The SON is one of the neural pathways involved in the control of ovarian functions, including puberty onset [3], ovulation [4-6] steroidogenesis, and compensatory ovarian hypertrophy [7,8]. The SON originates at the celiac ganglion, penetrates into the ovary through the hilium, and innervates the ovarian stroma, particularly the theca and secondary interstitial cells. Both of these cells are responsible for androgen synthesis [1,9,10]. The sympathetic innervations * Correspondence: [email protected] 1 Biology of Reproduction Research Unit. Physiology of Reproduction Laboratory, Facultad de Estudios Superiores Zaragoza, UNAM. AP 9-020, CP15000, México, D.F, Mexico Full list of author information is available at the end of the article
reaching the rat’s ovary are formed of fibers containing catecholamines, neuropeptide Y (NPY), and vasoactive intestinal peptide (VIP). The fibers that transport catecholamines and VIP are associated with steroidogenic tissue; while the fibers that transport NPY are associated with blood vessels and ovarian interstitial ti
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