Efficacy of antimicrobial agents delivered to hernia meshes using an adaptable thermo-responsive hyaluronic acid-based c
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ORIGINAL ARTICLE
Efficacy of antimicrobial agents delivered to hernia meshes using an adaptable thermo‑responsive hyaluronic acid‑based coating B. Pérez‑Köhler1,4,5 · F. Linardi2 · G. Pascual1,4,5 · J. M. Bellón3,4,5 · D. Eglin2 · O. Guillaume2 Received: 24 July 2019 / Accepted: 17 November 2019 © Springer-Verlag France SAS, part of Springer Nature 2019
Abstract Purpose Mesh-related infection is a critical outcome for patients with hernia defect stabilized with synthetic or biological meshes. Even though bioactive meshes loaded with antibiotics or antiseptics are slowly emerging in the market, the available solutions still lack versatility. Here, we proposed a polymer solution, i.e., hyaluronic acid-poly(N-isopropylacrylamide) (HApN), which forms a hydrogel to be used as coating for meshes only when it reaches body temperature. Methods We assessed how the gelation of HApN was influenced by the incorporation of different antibiotic and antiseptic formulations, and how this gel can be used to coat several mesh types. The impact of the coating on the elastic behavior of a macroporous mesh was tested under cyclic elongation condition. Finally, we selected two different coating formulations, one based on antibiotics (gentamicin + rifampicin) and one based on antiseptic (chlorhexidine) and tested in vitro their antimicrobial efficacies. Results HApN can be used as carrier for different antimicrobial agents, without having a strong influence on its gelation behavior. Porous or dense meshes can be coated with this polymer, even though the stability was not optimal on macroporous meshes such as Optilene when pores are too large. HApN loaded with drugs inhibited in vitro the growth of several Grampositive and Gram-negative bacteria. Conclusion Compared to the available technologies developed to endow meshes with antibacterial activity, the proposed HApN offers further versatility with potential to prevent mesh-related infection in hernioplasty. Keywords Antibacterial · Coating · Hernia mesh · Hydrogel · Infection · Thermo-responsive
Introduction Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10029-019-02096-3) contains supplementary material, which is available to authorized users. * O. Guillaume [email protected] 1
Department of Medicine and Medical Specialities, Faculty of Medicine and Health Sciences, University of Alcalá, Madrid, Spain
2
AO Research Institute Davos, 7270 Davos, Switzerland
3
Department of Surgery, Medical and Social Sciences, Faculty of Medicine and Health Sciences, University of Alcalá, Madrid, Spain
4
Biomedical Networking Research Centre On Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Madrid, Spain
5
Ramón y Cajal Health Research Institute (IRYCIS), Madrid, Spain
The utilization of mesh materials in the field of abdominal wall hernia repair has brought unquestionable clinical advantages over the last 5 decades. Nevertheless, mesh implantation can result in different postoperative complicati
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