Enhanced enzymatic removal of anthracene by the mangrove soil-derived fungus, Aspergillus sydowii BPOI
- PDF / 861,677 Bytes
- 13 Pages / 595.276 x 785.197 pts Page_size
- 79 Downloads / 189 Views
RESEARCH ARTICLE
Enhanced enzymatic removal of anthracene by the mangrove soil-derived fungus, Aspergillus sydowii BPOI Paul Olusegun Bankole (✉)1, Kirk Taylor Semple2, Byong-Hun Jeon3, Sanjay Prabhu Govindwar3 1 Department of Pure and Applied Botany, College of Biosciences, Federal University of Agriculture Abeokuta, Ogun State 234039, Nigeria 2 Lancaster Environment Centre, Lancaster University, Lancaster LA1 4YQ, UK 3 Department of Earth Resources and Environmental Engineering, Hanyang University, Seoul 04763, Republic of Korea
HIGHLIGHTS
GRAPHIC ABSTRACT
• A. sydowii strain bpo1 exhibited 99.8% anthracene degradation efficiency. • Four unique metabolic products were obtained after anthracene degradation. • Ligninolytic enzymes induction played vital roles in the removal of anthracene. • Laccase played a crucial role in comparison with other enzymes induced.
ARTICLE INFO Article history:
Received 27 July 2020 Revised 23 September 2020 Accepted 30 September 2020 Available online 5 November 2020 Keywords: Aspergillus sydowii Anthracene Biodegradation Polycyclic aromatic hydrocarbons
ABSTRACT The present study investigated the efficiency of Aspergillus sydowii strain bpo1 (GenBank Accession Number: MK373021) in the removal of anthracene (100 mg/L). Optimal degradation efficiency (98.7%) was observed at neutral pH, temperature (30°C), biomass weight (2 g) and salinity (0.2% w/v) within 72 h. The enzyme analyses revealed 131%, 107%, and 89% induction in laccase, lignin peroxidase, and manganese peroxidase respectively during anthracene degradation. Furthermore, the degradation efficiency (99.8%) and enzyme induction were significantly enhanced with the addition of 100 mg/L of citric acid and glucose to the culture. At varying anthracene concentrations (100– 500 mg/L), the degradation rate constants (k1) peaked with increasing concentration of anthracene 2 while the half-life (t1/2) decreases with increase in anthracene concentration. Goodness of fit (R = 0.976 and 0.982) was observed when the experimental data were subjected to Langmuir and Temkin models respectively which affirmed the monolayer and heterogeneous nature exhibited by A. sydwoii cells during degradation. Four distinct metabolites; anthracene-1,8,9 (2H,8aH,9aH)-trione, 2,4adihydronaphthalene-1,5-dione, 1,3,3a,7a-tetrahydro-2-benzofuran-4,7-dione and 2-hydroxybenzoic acid was obtained through Gas Chromatography-Mass spectrometry (GC-MS). A. sydowii exhibited promising potentials in the removal of PAHs. © Higher Education Press 2020
1
Introduction
Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous, persistent organic pollutants commonly found in air, water, and soil. They consist of carbon and hydrogen atoms fused with the aid of two or more aromatic pentacyclic or benzene rings (Baltrons et al., 2018, Wu et al., 2020). Pipeline vandalism, sabotage due to civil unrest, militancy
✉ Corresponding author E-mail: [email protected]
and war, oil spillage during exploration, over-exploitation, spillage from tankers during loading and transfers and
Data Loading...
