Environmental Regulation of Mitochondria-Rich Cells in Chalcalburnus tarichi (Pallas, 1811) During Reproductive Migratio
- PDF / 354,127 Bytes
- 6 Pages / 595.276 x 790.866 pts Page_size
- 11 Downloads / 132 Views
ORIGINAL ARTICLES
Environmental Regulation of Mitochondria-Rich Cells in Chalcalburnus tarichi (Pallas, 1811) During Reproductive Migration Ahmet R. Og˘uz
Received: 22 August 2012 / Accepted: 15 October 2012 / Published online: 3 November 2012 Ó Springer Science+Business Media New York 2012
Abstract Chalcalburnus tarichi is an anadromous cyprinid fish that has adapted to extreme conditions (salinity 22 %, pH 9.8 and alkalinity 153 mEq 9 l-1) in Lake Van in eastern Turkey. Changes in immunoreactivity of Na?/ K?-ATPase in gill tissue and osmolarity and ion levels in plasma were investigated in C. tarichi during reproductive migration. Physicochemical characteristics and ion levels in Lake Van were high compared freshwater. Plasma osmolality and plasma ion concentrations ([Na?], [K?] and [Cl-]) increased after transfer from freshwater to Lake Van. The mitochondria-rich (MR) cells of the gill were stained in both filament and lamellar epithelia of C. tarichi by immunocytochemistry with a specific antiserum for Na?/K?-ATPase in river fish samples. Density and area of MR cells were decreased in lake-adapted fishes. These results indicated that freshwater acclimation capacity is correlated with the size and distribution of MR cells in C. tarichi, in contrast to many teleost fishes. Keywords Mitochondria-rich cell Na?/K?-ATPase Osmoregulation Lake Van Chalcalburnus tarichi
Introduction Euryhaline fish possess the capacity to live at various salinity concentrations. Fishes are adapted to different salinity environments with a number of changes in osmoregulatory organs (Evans et al. 2005). Basic tissues in fish osmoregulation are gastrointestinal epithelium, kidney and A. R. Og˘uz (&) Department of Biology, Science Faculty, Yu¨zu¨ncu¨ Yıl University, 65080 Van, Turkey e-mail: [email protected]
gill. The gill is an important tissue in osmoregulation, acid base regulation and ion regulation and can contain three different cell types: mitochondria-rich (MR) or chloride cells, pavement cells and mucous-secreting cells. MR cells have Na?, K?, ATPase they are present in extensive tubular system continuous with the basolateral membrane and numerous mitochondria in cytoplasm (Wilson and Laurent 2002). Mucous cells serve to protect the gills against pathogenic microorganisms and parasitic infection with mucous secretion (Dezfuli et al. 2010). The MR cell has vital importance in osmoregulation due to transport of Na? and Cl- from the water into body or vice versa in different salinity environments. The MR cell displays a series of changes after adaptation to high-salinity environments. These changes are characterized by hypertrophy, increases basal–lateral cell surface area and Na?/K?-ATPase content and location in gills (Karnaky et al. 1976; Uchida et al. 1996; Hirai et al. 1999). Lake Van is the largest soda lake in the world, and Chalcalburnus tarichi is endemic to the lake. Extreme conditions are found in the lake such as high salinity (0.22 %), pH 9.8 and alkalinity (153 mEq 9 l-1) (Danulat and Kempe 1992). C. taric
Data Loading...
