Fine mapping of the peach pollen sterility gene ( Ps / ps ) and detection of markers for marker-assisted selection
- PDF / 491,133 Bytes
- 10 Pages / 547.087 x 737.008 pts Page_size
- 76 Downloads / 233 Views
Fine mapping of the peach pollen sterility gene (Ps/ps) and detection of markers for marker-assisted selection I Eduardo & C de Tomás & KG Alexiou & D Giovannini & M Pietrella & S Carpenedo & MC Bassols Raseira & I Batlle & CM Cantín & MJ Aranzana & P Arús
Received: 16 January 2020 / Accepted: 29 May 2020 # Springer Nature B.V. 2020
Abstract In peach, pollen sterility, expressed as absence of pollen in the anthers, segregates as an undesired
S. Carpenedo : . Bassols Raseira Embrapa Clima Temperado, Pelotas, RS 96010-971, Brazil
trait in breeding programs. Pollen fertility screening in progenies is not a common practice mainly because it does not affect fruit set since cross-pollination is frequent. It is also a time-consuming activity that coincides with the busy pollination season. Segregation for this trait could be avoided by using molecular markers to identify appropriate parents or male sterile plants for early culling in progenies expected to segregate, thus increasing breeding efficiency. In peach, pollen sterility is determined by a recessive allele in homozygosis of the major gene, Ps/ps, located on chromosome 6. In this work, using a conventional mapping approach combined with bulked segregant analysis using resequencing data, we fine mapped Ps to a region of almost 160 kb and developed molecular markers for marker-assisted breeding. These markers were validated in plant materials from three peach breeding programs, including progenies, advanced selections, and cultivars, allowing us to determine that the frequency of the ps allele is high (0.23) and also to infer the genotypes of a large collection of cultivars and advanced breeding lines.
I. Batlle Centre Mas de Bover, IRTA, Crta. Reus - El Morell Km 3.8. 43120 Constantí, Tarragona, Spain
Keywords Prunus persica . Male sterility . Markerassisted breeding . Breeding program
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11032-020-01139-3) contains supplementary material, which is available to authorized users. I. Eduardo : C. de Tomás : K. Alexiou : M. Aranzana : P. Arús IRTA, Institut de Recerca i Tecnologia Agroalimentàries, Barcelona, Spain I. Eduardo (*) : C. de Tomás : K. Alexiou : M. Aranzana : P. Arús Centre for Research in Agricultural Genomics (CRAG) CSIC-IRTA-UAB-UB, Campus UAB, Cerdanyola del Vallès, Bellaterra, 08193 Barcelona, Spain e-mail: [email protected] D. Giovannini : M. Pietrella Council for Agricultural Research and Economics, Research Centre for Olive, Fruit and Citrus Crops, CREA, Forlì, Italy
C. Cantín IRTA. FruitCentre. Parc Cientific i Tecnològic Agroalimentari de Lleida (PCiTAL), Edifici Fruitcentre, Parc de Gardeny, 25003 Lleida, Spain C. Cantín ITA-ARAID. Agrifood Research and Technology Centre of Aragón, Avda. Montañana 930, 50059 Zaragoza, Spain
Introduction Male sterile individuals are sometimes found in segregating progenies of peach [Prunus persica L. (Batsch.)]. Flowers from male sterile individuals have no pollen,
57
Page 2 of 10
and the anthers are small
Data Loading...
