Genistein contributes to cell cycle progression and regulates oxidative stress in primary culture of osteoblasts along w
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(2020) 20:277
RESEARCH ARTICLE
BMC Complementary Medicine and Therapies
Open Access
Genistein contributes to cell cycle progression and regulates oxidative stress in primary culture of osteoblasts along with osteoclasts attenuation Sahabjada Siddiqui1,2* , Abbas Ali Mahdi3 and Md Arshad2,4*
Abstract Background: The present study was designed to examine the role of isoflavone genistein (GS) on bone formation, regulating oxidative stress and cell cycle in primary osteoblasts, as well as attenuation of osteoclast formation. Methods: Primary calvaria osteoblasts were isolated from 2 to 3 days old neonatal rat pups (n = 6–8) of Sprague Dawley rats. Osteoblasts were incubated with varying concentrations of GS and different assays viz. cell proliferation, differentiation, calcium deposition, cell cycle progression, antioxidant ability, and osteogenic gene expression were performed. Tartrate-resistant acid phosphatase (TRAP) staining and immunolocalization of cathepsin K protein were assessed in bone marrow-derived osteoclasts. Results: Results revealed that GS markedly induced cell growth and osteoblast differentiation depending upon dose. The fluorescent dye DCFH-DA staining data proved the antioxidant ability of GS, which reduced the H2O2induced intracellular oxidative stress in osteoblasts. Quantitative real-time PCR analysis revealed that GS treatment upregulated the expression of osteoblastic genes of Runt-related transcription factor 2 (Runx2), bone morphogenetic proteins 2 (BMP2), and osteocalcin. Immunolocalization of BMP2 also indicated the osteogenic efficacy of GS. Furthermore, TRAP staining and cathepsin K expression depicted that GS inhibited multinucleated osteoclasts formation. Conclusions: In conclusion, GS isoflavone might impart protective effects against oxidative stress-induced bone loss and thus, could maintain skeletal growth. Keywords: Cell cycle, Isoflavone, Oxidative stress, Primary bone cells
Background The balance between bone formation, bone resorption, proliferation, and apoptosis helps to maintain bone mass nearly constant [1]. An imbalance between bone resorption and bone formation may cause metabolic disorders * Correspondence: [email protected]; [email protected]; [email protected] 1 Department of Biotechnology, Era’s Lucknow Medical College & Hospital, Era University, Lucknow 226003, India 2 Department of Zoology, University of Lucknow, Lucknow 226007, India Full list of author information is available at the end of the article
or bone pathological conditions of the adult skeleton and lead to low bone mass, osteoporosis, and increased bone fracture risk [2]. Based on available data and clinical experiences, about 200 million women worldwide are osteoporotic and report approximately 8.9 million fractures annually [3]. This is equivalent to an osteoporotic fracture every 3 s in the woman [4]. The most common risk factors for osteoporotic fracture are deteriorating bone mineral density along with advanced age [5]. Therefore, diet strategies, including funct
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