Helicobacter genus in the intestine and liver of stray cats: the molecular, histopathological, and immunohistochemical s
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VETERINARY MICROBIOLOGY - RESEARCH PAPER
Helicobacter genus in the intestine and liver of stray cats: the molecular, histopathological, and immunohistochemical study Boshra Elyasi 1 & Annahita Rezaie 2
&
Naghmeh Moori Bakhtiari 2 & Bahman Mosallanejad 3
Received: 21 April 2020 / Accepted: 4 August 2020 # Sociedade Brasileira de Microbiologia 2020
Abstract Objectives The study was designed to determine the presence of Helicobacter genus and three species of H. pylori, H. bilis, and H. canis, in the duodenum, ileum, colon, and liver of stray cats. Moreover, the histopathological and immunohistochemical analyses have been performed. Methods Samples were taken from the duodenum, ileum, colon, and liver of 30 cats for molecular and histopathological evaluations. Polymerase chain reaction was carried out for the detection of the Helicobacter genus in the mentioned samples. Then, species-specific primers were used in Helicobacter-positive samples. Results Helicobacter genus prevalence rates in the duodenum, ileum, colon, and liver samples were 50%, 60%, 50%, and 43.3%, respectively. Helicobacter pylori, H. canis, and H. bilis were isolated from at least one tissue of 18 (60%), 13 (43.3%), and 8 (26.7%) of the cats, respectively. Immunohistochemical findings confirmed the presence of bacteria in the intestinal crypt or the mucosal layer of duodenum, ileum, colon, and hepatic sinusoids. Conclusion In the present study, the concurrent infection of duodenum and liver was noticeable. Furthermore, the high prevalence of H. pylori in cats, as a well-known human pathogen, should be considered. High incidence of Helicobacter in gut and liver of Ahvaz stray cats is noticeable. According to the zoonotic importance of Helicobacter, more studies in the field of treatment and prevention are highly recommended. Keywords Cat . Helicobacter pylori . Helicobacter bilis . Helicobacter canis . Intestine . Liver
Introduction Helicobacter genus is the most important bacteria in the gastrointestinal tract of humans and animals. Helicobacters are gram-negative, microaerophilic, and curved to spiral-shaped bacteria of Helicobacteraceae family and Helicobacter genus [13]. Forty-five species of Helicobacter have been detected by Responsible Editor: Miliane Moreira Soares de Souza. * Annahita Rezaie [email protected]; [email protected] 1
Graduated in the doctorate of Veterinary Medicine, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
2
Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Golestan Bulvar, Ahvaz, Iran
3
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
Polymerase chain reaction (PCR) in fecal samples from 150 vertebrates so far [5]. Previous researches have shown the existence of the Helicobacter genus in the gastric mucosa of the cat [2, 3, 6, 9, 25, 28]. A few studies have focused on the detection of Helicobacter in other parts of the gastrointestinal system or feces. For example,
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