Identification of qLG2 , qLG8 , and qWG2 as novel quantitative trait loci for grain shape and the allelic analysis in cu
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ORIGINAL ARTICLE
Identification of qLG2, qLG8, and qWG2 as novel quantitative trait loci for grain shape and the allelic analysis in cultivated rice Gao‑Jie Wang1,3 · Ying Wang1,3 · Jie‑Zheng Ying2 · Xian‑Jun Song1,3,4 Received: 19 March 2020 / Accepted: 8 July 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Main conclusion Three novel QTLs for grain shape were genetically fine mapped, with two of which to a 250-kb target interval on rice chromosome 2 that contains fourteen candidate genes. Abstract Grain shape (grain length, width, and thickness) determines crop yield and grain quality. However, the trait is regulated by numerous naturally occurring quantitative trait loci (QTLs) and the underlying mechanism remains largely unknown. Here, we report the genetic mapping of three new QTLs, qLG2, qWG2, and qLG8 that each exerts a semi-dominant effect on grain shape in cultivated rice. These QTLs were validated using populations derived from the corresponding chromosome segment substitution lines (CSSLs), and were further delimited to small genomic intervals in progeny testing experiments. Especially, qLG2/qWG2 was placed into an about 250-kb genomic candidate region, and 14 predicted ORFs localized within the interval. We also evaluated the individual and pyramiding genetic effect(s) of these QTL(s) using the corresponding nearly isogenic lines, and found that they have additive effects on the traits. Collectively, these findings provided useful information as a tool to improve grain shape in crop breeding programs and established foundations for future QTL cloning. Keywords Grain length · Grain width · Grain shape · QTL mapping · Rice plant Abbreviations CSSL Chromosome segment substitution line NIL Near isogenic line
QTL Quantitative trait locus SSR Simple sequence repeat
Introduction Communicated by Dorothea Bartels. Gao-Jie Wang and Ying Wang contributed equally. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00425-020-03420-3) contains supplementary material, which is available to authorized users. * Xian‑Jun Song [email protected] 1
Key Laboratory of Plant Molecular Physiology, Institute of Botany, The Chinese Academy of Sciences, No. 20 Nanxincun, Xiangshan, Beijing 100093, China
2
National Key Laboratory of Rice Biology and Chinese Center of Rice Improvement, China National Rice Research Institute, Hangzhou 310006, China
3
Graduate University of Chinese Academy of Sciences, Beijing, China
4
The Innovative Academy of Seed Design, The Chinese Academy of Sciences, Beijing, China
Rice (Oryza sativa L.) is an important crop plant that meets the food demands of over half of the world population (Khush 2001; Wang and Li 2011). Given that world population is ever growing and there are still various unfavorable factors to threaten crop production, it is crucial and essential to develop rice varieties with higher productivity through efficient breeding (Godfray et al. 2010). Grain shape (grain length, width and t
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