Identification, tissue distribution, periprandial expression , and anorexigenic effect of spexin in Siberian sturgeon, A
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Identification, tissue distribution, periprandial expression, and anorexigenic effect of spexin in Siberian sturgeon, Acipenser baeri Zhengzhi Tian & Shaoqi Xu & Mei Wang & Ya Li & Hu Chen & Ni Tang & Bin Wang & Xin Zhang & Zhiqiong Li
Received: 8 April 2020 / Accepted: 4 August 2020 # Springer Nature B.V. 2020
Abstract Spexin (Spx), an endogenous peptide, is considered to be a neuropeptide. In a few fish and mammals, it has been proved to play a role in the regulation of animal feeding. However, the possible mechanisms of spexin regulating food intake are mostly blurry in vertebrates including Siberian sturgeon. In this study, firstly, the coding sequence of spexin cDNA was cloned and sequenced in Siberian sturgeon. Then, we detected that spexin
Highlights • The partial cDNA sequence and tissue distribution of Siberian sturgeon spexin gene were shown. • The mRNA expression of spexin increased after feeding in Siberian sturgeon hypothalamus. • Intraperitoneal injection with spexin reduced the food intake of Siberian sturgeon and changed the mRNA expression of some appetite-regulating factors in both hypothalamus and stomach. Zhengzhi Tian, Shaoqi Xu, Mei Wang, and Ya Li paid a similar amount of work and carried out experiments and thesis writing. Zhengzhi Tian, Shaoqi Xu, Mei Wang, Ya Li, Ni Tang, and Bin Wang participated in animal experiments. All authors participated in experimental design and thesis writing. Z. Tian : S. Xu : M. Wang : Y. Li : H. Chen : N. Tang : B. Wang : Z. Li (*) Department of Aquaculture, College of Animal Science and Technology, Sichuan Agricultural University, 211# Huimin Road, Chengdu, Sichuan, China e-mail: [email protected] X. Zhang The Key Laboratory of Mariculture, Ministry of Education, Fisheries College, Ocean University of China, 5# Yushan Road, Qingdao, Shandong, China
mRNA was widely expressed in the hypothalamus, gastrointestinal tract, and liver, with the highest expression in the hypothalamus. The expression of spexin mRNA in the hypothalamus was significantly increased after food intake. At 1 h, 3 h, and 6 h after injection, the food intake in the spexin group (0.10, 0.30, and 0.90 μg/g BW) was significantly lower than that in the saline group. Moreover, compared with the saline group, the mRNA expression of anorectic nucb2, cart, ucn3, and pyy in the hypothalamus was significantly upregulated and orectic npy was significantly downregulated at 1 h after spexin injection; in the stomach, the mRNA expression of nucb2 and pyy was significantly upregulated. All in all, these results provide evidence for the anorexic effect of spexin on Siberian sturgeon.
Keywords Food intake . Feeding status . Appetiteregulating peptide . Spexin . Siberian sturgeon Abbreviations nucb2 Nucleobindin-2 cart Cocaine-and amphetamine-regulated transcript ucn3 Urocortin 3 pyy Peptide yy cck Cholecystokinin npy Neuropeptide y qPCR Real-time fluorescence quantification PCR ORF Open reading frame
Fish Physiol Biochem
Introduction Food intake is one of the important ways for animals to obtain e
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