Influence of Trace Elements on Stabilization of Aqueous Solutions of Ascorbic Acid
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Influence of Trace Elements on Stabilization of Aqueous Solutions of Ascorbic Acid Barbara Dolińska & Aneta Ostróżka-Cieślik & Artur Caban & Klimas Rimantas & Lucyna Leszczyńska & Florian Ryszka
Received: 29 May 2012 / Accepted: 5 October 2012 / Published online: 26 October 2012 # The Author(s) 2012. This article is published with open access at Springerlink.com
Abstract Together with vitamin C, zinc, selenium, manganese, and magnesium play a vital role in the preservation of organs scheduled for transplantation. In the present study, it is shown that addition of 1 mg/l of these elements influences the stability of 0.3 mM ascorbic acid solutions. The solution’s stability was estimated using an accelerated stability test. The concentration of vitamin C was measured using a validated spectrophotometric method, which uses the reduction of 2,6-dichlorophenoloindophenol by ascorbic acid. Elevated temperatures, the factor accelerating substances’ decomposition reaction rate, were used in the tests. The research was conducted at two temperatures at intervals of 10 °C: 80±0.1 and 90±0.1 °C. It was stated that the studied substances’ decomposition occurred in accordance with the equation for first-order reactions. The function of the logarithmic concentration (log%C) over time was revealed to be rectilinear. This dependence was used to determine the kinetics of decomposition reaction rate parameters. The stabilization of vitamin C solutions was measured as the time in which 10 % of the substance decomposed at 20 and B. Dolińska (*) : L. Leszczyńska : F. Ryszka Biochefa Pharmaceutical Research and Production Plant, Sosnowiec, Poland e-mail: [email protected] B. Dolińska : A. Ostróżka-Cieślik Department of Applied Pharmacy and Drug Technology, Silesian Medical Academy, Katowice, Poland A. Caban Department of General, Vascular and Transplant Surgery, Medical University of Silesia Katowice, Katowice, Poland K. Rimantas Department of Drug Technology, Kaunas University of Medicine, Kaunas, Lithuania
0 °C. Addition of Se(IV) or Mg(II) ions significantly increase the stability of ascorbic acid solution (∼34 and ∼16 %, respectively), but Zn(II) causes a significant decrease in stability by ∼23 %. Addition of Mn(II) has no significant influence on vitamin C stability. Keywords Microelements . Vitamin C . Solutions . Preservation . Accelerated stability test
Introduction Trace elements are essential for appropriate function of living organisms. Some elements can have a protective influence on selected organs during ischemia and reperfusion [1–6] and might have a positive role in stabilization of organ preservation solutions [7–9]. One such element is selenium, a component of glutathione peroxidase that plays a protective role against the oxidizing action of hydrogen peroxide [1–4]. Zinc influences cell membrane structure and function. It is a component of several enzymes and stimulates protein synthesis. It is a component of superoxide dismutase (Cu/Zn-SOD) and takes part in quenching of superoxide radicals. It was concluded that zinc is a
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