Lasiodiplodia pseudotheobromae causes stem canker of Chinese hackberry in China
- PDF / 2,145,740 Bytes
- 10 Pages / 595.276 x 790.866 pts Page_size
- 12 Downloads / 143 Views
ORIGINAL PAPER
Lasiodiplodia pseudotheobromae causes stem canker of Chinese hackberry in China Lu Liang1 · Huan Li1 · Lifeng Zhou1 · Fengmao Chen1
Received: 28 March 2019 / Accepted: 24 June 2019 © The Author(s) 2019
Abstract Chinese hackberry (Celtis sinensis Pers.) is an adaptable species widely growing in southern China. The symptoms of canker on stems of seedlings were discovered mid-July 2017 in Shuyang, Jiangsu Province. The diseased portions of the stems were dark brown due to discoloured xylem. Some seedlings showed symptoms of wilting, leaf fall, twig dieback, and tissue discolouration. The outbreak period was concentrated in July and August, suggesting that the disease spread during summer months. Possible fungal causal agents were isolated from naturally infected canker tissue and discoloured xylem. The isolate from xylem tissue with a high frequency (> 50%) was named Ls7 type. Pathogenicity tests were carried out on 4-yearold seedlings. The symptoms of canker began to develop 20 days after inoculation with Ls7 isolate and by day 35, there were dark, enlarged longitudinal lesions. A phylogenetic tree of the isolate was developed using the internal transcribed spacer, elongation factor-1α (tef1-α), β-tubulin gene (TUB) and RNA polymerase II subunit primer genes (RPB2). Based on morphological features and phylogenetic information, the pathogen was identified as Lasiodiplodia
Project funding: The work was supported by the National Key Research and Development Program of China (Grant Number: 2017YFD0600104). The online version is available at http://www.springerlink.com. Corresponding editor: Tao Xu. * Fengmao Chen [email protected] 1
Co‑Innovation Center for Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037, Jiangsu, People’s Republic of China
pseudotheobromae. This is the first report of L. pseudotheobromae causing canker on Chinese hackberry stems in China. Keywords Botryosphaeriaceae · Plant pathogen · Tree disease
Introduction Chinese hackberry (Celtis sinensis Pers.) is an important and widely planted ornamental tree species in China, Japan and Korea (Lee et al. 2019) and is tolerant of dry and nutrientpoor soils. The tender leaf, roots and bark of hackberry can reduce swelling, relieve pain, clearing away heat and toxic materials. It is also used as industrial materials and various furniture. Its rhizome fibers are strong and used in paper making, making ropes, artificial cotton and artificial fiber. The species has significant value in medicine and industry (Cao 2017). However, with increased planting, Chinese hackberry diseases are increasing and becoming more and more serious (Cacciola 2000; Berbegal et al. 2012; Luongo et al. 2015). Common diseases of Chinese hackberry are powdery mildew, stem and root rot, twig blight and leaf spot. The Botryosphaeriaceae is a fungal family containing pathogens that cause canker disease of trees (Pavlic 2004). Lasiodiplodia species are members of the Botryosphaeriaceae and are widely found i
Data Loading...
