Local application of curcumin-loaded nanoparticles as an adjunct to scaling and root planing in periodontitis: Randomize
- PDF / 563,167 Bytes
- 11 Pages / 595.276 x 790.866 pts Page_size
- 74 Downloads / 159 Views
ORIGINAL ARTICLE
Local application of curcumin-loaded nanoparticles as an adjunct to scaling and root planing in periodontitis: Randomized, placebo-controlled, double-blind split-mouth clinical trial Cindy Grace Pérez-Pacheco 1 & Natalie Ap. Rodrigues Fernandes 1 & Fernando Lucas Primo 2 & Antonio Claudio Tedesco 3 & Emily Bellile 4 & Belen Retamal-Valdes 5 & Magda Feres 5 & Morgana Rodrigues Guimarães-Stabili 1 & Carlos Rossa Jr 1 Received: 18 June 2020 / Accepted: 20 October 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Objective Assess a single local application of curcumin-loaded nanoparticles as an adjunct to scaling and root planing (SRP) in nonsurgical periodontal treatment (NPT). Materials and methods Twenty healthy subjects with periodontitis received SRP+PLGA/PLA nanoparticles loaded with 50 μg of curcumin (N-Curc) or SRP+empty nanoparticles. Probing pocket depth (PPD), clinical attachment level (CAL), and bleeding on probing (BOP) were monitored at baseline, 30, 90, and 180 days. IL-1α, IL-6, TNFα, and IL-10 in the gingival crevicular fluid (GCF) were assessed by ELISA, and counts of 40 bacterial species were determined by DNA hybridization at baseline, 3, 7, and 15 days post-therapy. Results PPD, CAL, and BOP were similarly and significantly improved in both experimental groups. There was no difference in GCF cytokine levels between experimental groups, although IL-6 was decreased at 3 days only in the N-Curc group. NPT reduced counts of red complex bacterial species in both groups. Veillonella Parvula counts increased significantly only in N-Curc group at 7 days, whereas Aggregatibacter actinomycetemcomitans counts increased significantly only in the control group from day 3 to day 15. Conclusion We conclude that a single local administration of nanoencapsulated curcumin in periodontally diseased sites had no additive benefits to NPT. Clinical relevance Our results showed that a single local application of curcumin-loaded nanoparticles associated with nonsurgical periodontal therapy did not improve clinical outcomes. Hence, our findings do not support the use of curcumin as an adjunct to nonsurgical periodontal therapy. Keywords Curcumin . Periodontitis . Dental Scaling . Nanoparticles
Introduction Periodontitis is a chronic inflammatory condition by destruction of tooth-supporting tissues [44]. Periodontitis was * Carlos Rossa, Jr [email protected] 1
2
Department of Diagnosis and Surgery, School of Dentistry at Araraquara, Sao Paulo State University (UNESP), Araraquara, SP, Brazil Department of Bioprocess and Biotechnology, Faculty of Pharmaceutical Sciences of Araraquara, Sao Paulo State University (UNESP), Araraquara, SP, Brazil
considered the 6th most prevalent chronic inflammatory condition worldwide in 2010 [30]. Disease is initiated by a dysbiosis between host and microorganisms of the dental biofilm [33, 58]. The standard therapy 3
Department of Chemistry, Center of Nanotechnology and Tissue Engineering- Photobiology and Photomedicine Research Group, Faculty o
Data Loading...
