Long Non-coding RNA Expression Profile in Broiler Liver with Cadmium-Induced Oxidative Damage
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Long Non-coding RNA Expression Profile in Broiler Liver with Cadmium-Induced Oxidative Damage Chunlin Yu 1,2 & Chaowu Yang 1 & Xiaoyan Song 1 & Jingjing Li 2 & Han Peng 1 & Mohan Qiu 1 & Li Yang 1 & Huarui Du 1 & Xiaosong Jiang 1 & Yiping Liu 2 Received: 28 July 2020 / Accepted: 11 October 2020 # Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Cadmium pollution is serious heavy metal pollution in environmental pollution and impacts on livestock productivity. However, the effect and mechanisms of cadmium toxicity on the broiler remain unclear. This study aimed to explore the liver oxidative damage and reveal the related long non-coding RNA (lncRNA) expression patterns in the broiler liver with cadmium exposure. The broilers were fed with diets containing CdCl2 and detected the oxidative stress indexes in the liver tissues. Transcriptome sequencing of broiler liver was performed to identify cadmium exposure-related differentially expressed lncRNAs (DElncRNAs). The functions and pathways of DElncRNAs were analyzed by GO and KEGG. The sequencing results were verified by the quantitative real-time polymerase chain reaction. Cadmium exposure induced tissue structure disorder, focal hemorrhage, and irregular hepatocytes in the broiler liver, and significantly decreased GSH level and enzyme activities, and increased MDA expression in the liver. A total of 74 DElncRNAs were obtained in cadmium group compared with the control group, which were enriched in the GO terms, including intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator, branched-chain amino acid biosynthetic process. The enriched KEGG pathways, including lysine biosynthesis, valine, leucine and isoleucine biosynthesis, and pantothenate and CoA biosynthesis, were related to oxidative stress. PCR analysis indicated that the changes in ENSGALG00000053559, ENSGALG00000053926, and ENSGALG00000054404 expression were consistent with sequencing. Our results provide novel lncRNAs involved in oxidative stress in the broiler liver with cadmium exposure. Keywords Cadmium toxicity . Broiler liver . Organ toxicity . Antioxidant defense system enzymes
Introduction Cadmium (Cd) is a kind of unessential element of the human body, and its accumulation leads to toxin in all forms of life Chunlin Yu and Chaowu Yang contributed equally to this work. Chunlin Yu and Chaowu Yang are equal contributors. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s12011-020-02436-w) contains supplementary material, which is available to authorized users. * Yiping Liu [email protected] 1
Animal Breeding and Genetics key Laboratory of Sichuan Province, Sichuan Animal Science Academy, Chengdu 610066, Sichuan, China
2
Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Huimin Road, Wenjiang District, Chengdu 611130, Sichuan Province, China
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