Management of urinary and bowel dysfunction in rabbit model of spinal cord injury using Schwann cells and muscle progeni
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UROLOGY - ORIGINAL PAPER
Management of urinary and bowel dysfunction in rabbit model of spinal cord injury using Schwann cells and muscle progenitors: functional study and evidence for novel mechanism of action Shabnam Sabetkish1 · Reza Nejad Gashti1 · Bahareh Mohammadi Jobani1 · Maryam Alijani1 · Maryam Farsi1 · Shaghayegh Mousavi1 · Alireza Moradzadeh1 · Jaleh Parizad1 · Masoumeh Majidi Zolbin1 · Abdol‑Mohammad Kajbafzadeh1 Received: 8 September 2020 / Accepted: 13 November 2020 © Springer Nature B.V. 2020
Abstract Purpose We tried to investigate the role of Schwann and satellite cells in the treatment of neurogenic bladder and bowel dysfunction; following spinal cord injury in the rabbit model. Methods Twelve male New Zealand rabbits underwent induction of neurogenic bladder by spinal cord injury. Rabbits underwent the fiber tractography analysis to confirm the induction of spinal cord injury. Then, animals were randomly divided into two groups. In group I (n = 4), Schwann cells were obtained from autologous peroneal nerve. In group II (n = 4), the co-culture of nerve-muscle cells was obtained from autologous peroneal nerve and quadriceps muscle. Animals in the control group (n = 4) did not undergo any rehabilitation therapy. One and 4 months after injection of cells into the external anal sphincter, electromyography, urethral pressure profiles, urodynamic studies, voiding cystourethrogram, and manometry was performed to confirm the efficacy of treatment in short- (1 month) and long-term (4 months) follow-ups. Results The investigations validated that no statistically significant difference was detected between the two experimental groups in a short-term follow-up (p-value > 0.05). However, the functional features were improved in group II in long-term follow-up. In both groups, the external anal sphincter contracted in response to electrical signals delivered to the muscle. However, more signals were detected in group II in electromyography evaluation. The immunohistochemical staining demonstrated that the histological features of the bladder and spinal cord were more satisfactory in group II in all follow-ups compared to group I, in terms of less edema, inflammation, presence of progenitor cells, and expression of muscle and nerve markes. Conclusion Our results suggested that the injection of nerve-muscle co-culture cells into the external anal sphincter may be a helpful tactic for ameliorating the urological complications; following spinal cord injury induction in the rabbit model. Keywords Tissue-engineering · Cell culture · Anal sphincter · Animal model
Introduction Supplementary Information The online version contains supplementary material available at https://doi.org/10.1007/s1125 5-020-02722-5. * Abdol‑Mohammad Kajbafzadeh [email protected] 1
Pediatric Urology and Regenerative Medicine Research Center, Section of Tissue Engineering and Stem Cells Therapy, Children’s Hospital Medical Center, Tehran University of Medical Sciences, No. 62, Dr. Gharib’s Street, Keshavarz Boulevard, T
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