Microfabricated, Wireless, Magnetoelastic Micro-Particles for the Detection of Bacillus Anthracis Spores
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0951-E06-31
MICROFABRICATED, WIRELESS, MAGNETOELASTIC MICRO-PARTICLES FOR THE DETECTION OF BACILLUS ANTHRACIS SPORES Jiehui Wan1,2, Michael L. Johnson1,2, Huihua Shu3, Valery A. Petrenko2,4, and Bryan A. Chin1,2 1 Material Engineering, Auburn University, 275 Wilmore Laboratory, Auburn, AL, 36849 2 Auburn University Detection and Food Safety center, Auburn, AL, 36849 3 Electrical Engineering, Auburn University, Auburn, AL, 36849 4 Department of Pathobiology, Auburn University, Auburn, AL, 36849 ABSTRACT Magnetoelastic resonance biosensors were fabricated by immobilizing a bio-molecular recognition element onto the surface of Fe79B21 magnetoelastic particles (MEP). These sensors can be measured wirelessly and remotely for both in-air and in-liquid bacteria detection. Filamentous bacterio-phage that was selected specifically for the detection of Bacillus anthracis spores was employed as the biomolecular recognition element and immobilized onto the MEPs’ surfaces. Attachment of the spores to the sensor surface due to specific phage-spore binding results in a shift in the resonance frequency of the biosensor. Insitu measurement of the resonance frequency of biosensors of 5x100x500 microns were used to determine the sensor response as a function of spore concentrations of 103 to 108 cfu/ml. Specificity of the sensor was evaluated by conducting tests using a mixture of Bacillus anthracis Sterne strain, Bacillus cereus and Bacillus megaterium spores. INTRODUCTION Rapid, sensitive, specific detection methods against biological warfare have long been a subject of research. Among all the current detection systems, immunoassay- and biosensor-based detection systems would be the best prospects since they require only a simple sensor platform and a specific, selective, and stable diagnostic probe for the target pathogen. These biosensor systems usually consist of two components: a bio-receptor that possesses affinity for a target pathogen, and a transducer which can convert the chemical signal of sample-receptor interaction to a functional signal output. Mass-sensitive acoustic wave biosensors are one of the most popular platforms investigated in this area [1,2]. Recently a new format of acoustic wave devices which utilizes magnetoelastic materials as transducers has been studied widely. This type of sensor has a simple structure and the fabrication process is relatively easy. Many magnetoelastic materials are very inexpensive, which reduces the costs of sensors and allows them to be used in a disposable manner. The most important feature of magnetoelastic material-based sensors is that the signal transmission is totally wireless, allowing use of these sensors remotely for continuous monitoring both in air and in liquid. Magnetoelastic sensors based upon amorphous metallic ribbon material in millimeter scale have been successfully applied to physical, chemical and biological sensing area [3-5]. In order to test for a small number of cells, or a low concentration of analytes, magnetoelastic micro-particles were fabricated using m
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