Multi-locus sequence typing of Mycoplasma bovis to assess its genetic diversity from 2009 to 2018 in Ningxia Hui Autonom
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RESEARCH ARTICLE
Open Access
Multi-locus sequence typing of Mycoplasma bovis to assess its genetic diversity from 2009 to 2018 in Ningxia Hui Autonomous Region, China Yanan Guo1, Haifeng Luo1, Shuqiang Guo2, Yuanyuan Lei3, Yong Li4 and Shenghu He1*
Abstract Background: Mycoplasma bovis (M. bovis) is a highly contagious cattle pathogen spreading worldwide and especially in Ningxia Hui Autonomous Region in China. Results: Two types of ST, ST10and ST134, were identified in Ningxia Hui Autonomous Region. Thirty-seven strains belonged to ST10 and 28 strains belonged to ST134. ST134 was a new ST and first found in 2009 and was only widely distributed in Ningxia Hui Autonomous Region at present. The M. bovis ST10 was widely spread in many provinces in China and was widespread in Ningxia Hui Autonomous Region since 2010. It is speculated that the prevalence of M. bovis ST10 in Ningxia Hui Autonomous Region began in 2010. Conclusions: This study is the first report on the genetic diversity of M. bovis from 2009 to 2018 in Ningxia Hui Autonomous Region and provides the epidemiological information. These results may help further our understanding of the evolution of M. bovis and provide information that may be useful for the development of novel vaccines. Keywords: Multi-locus sequence typing, Mycoplasma bovis, genetic diversity, Ningxia Hui Autonomous Region
Background Mycoplasma bovis (M. bovis) is an important pathogen causing severe pneumonia, mastitis, and arthritis in the world. Especially, pneumonia caused by M. bovis has high morbidity and mortality. It is becoming one of the most widely recognized pathogens in the world[1, 2]. The pathogen is highly contagious and can spread rapidly throughout the herd. M. bovis can not only cause pneumonia, mastitis, arthritis, and otitis but also induce postpartum infection of the uterus with a mortality rate of 80%[3]. Since there are no effective vaccines and drugs to * Correspondence: [email protected] 1 Department of Veterinary Clinical Sciences, School of Agriculture, Ningxia University, 750021 Yinchuan, Ningxia, China Full list of author information is available at the end of the article
prevent and cure the disease caused by the pathogen, the incidence of the disease is on the rise[4, 5]. With the completion of whole-genome sequencing of M. bovis, a variety of highly repeatable molecular typing methods have been developed for molecular epidemiology and population structure research, including arbitrarily primed PCR (AP-PCR), random amplified polymorphic DNA (RAPD)[6], amplified fragment length polymorphism (AFLP)[7], pulsed-field gel electrophoresis (PFGE)[8], insertion sequence (IS)[9], variable number of tandem repeats (VNTR)[10, 11], multiple-locus variable-number tandem repeat (MLVA)[10] and multi-locus sequence typing (MLST)[5]. Although AP-PCR, RAPD, AFLP, and PFGE methods can obtain a large amount of genetic information, they are subjective in the analysis of DNA
© The Author(s). 2020 Open Access This article is licensed under a Creative Commons Attribution 4
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