Multiple Sclerosis Methods and Protocols
This detailed volume covers molecular biology, cellular biology, biomarkers, imaging, and neuropathological methods and techniques to explore multiple sclerosis (MS), with a special emphasis on disease models. With so much effort needed to elucidate basic
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Robert Weissert Editor
Multiple Sclerosis Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
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Multiple Sclerosis Methods and Protocols
Edited by
Robert Weissert University of Regensburg Regensburg, Germany
Editor Robert Weissert University of Regensburg Regensburg, Germany
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-2629-9 ISBN 978-1-4939-2630-5 (eBook) DOI 10.1007/978-1-4939-2630-5 Library of Congress Control Number: 2015945631 Springer New York Heidelberg Dordrecht London # Springer Science+Business Media New York 2016 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper Humana Press is a brand of Springer Springer Science+Business Media LLC New York is part of Springer Science+Business Media (www.springer.com)
Preface The book Multiple Sclerosis: Methods and Protocols contains 17 chapters with state-of-theart methods and techniques contributed by eminent scientists that will enable investigators to perform research for multiple sclerosis (MS). First of all, I want to thank John M. Walker (Hertfordshire, United Kingdom), the Series Editor of Methods in Molecular Biology, for giving me the opportunity to select methods and protocols that I consider to be very important for research in MS. Therefore this book has a personal touch. The book covers molecular biology, cellular biology, biomarkers, imaging, and neuropathological methods and techniques. Moreover, special emphasis is put on disease models. The authors for this book were selected based on themes and research records. Maja Jagodic and Pernilla Strindh (Stockholm, Sweden) give a state-of-the-art overview describing methods to clone genes in experimental populations that are relevant for MS. Holger Reichardt and Henrike J. Fischer (Go¨ttingen, German
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