Naegleria fowleri : differential genetic expression following treatment with Hesperidin conjugated with silver nanoparti
- PDF / 798,004 Bytes
- 8 Pages / 595.276 x 790.866 pts Page_size
- 93 Downloads / 156 Views
PROTOZOOLOGY - SHORT COMMUNICATION
Naegleria fowleri: differential genetic expression following treatment with Hesperidin conjugated with silver nanoparticles using RNA-Seq Ruqaiyyah Siddiqui 1 & Kavitha Rajendran 2 & Bahaa Abdella 2,3 & Qasim Ayub 4,5 & Shu Yong Lim 4 & Naveed Ahmed Khan 1 Received: 3 March 2020 / Accepted: 5 May 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Naegleria fowleri causes a deadly infection known as primary amoebic meningoencephalitis (PAM). To our knowledge, there are very few transcriptome studies conducted on these brain-eating amoebae, despite rise in the number of cases. Although the Naegleria genome has been sequenced, currently, it is not well annotated. Transcriptome level studies are needed to help understand the pathology and biology of this fatal parasitic infection. Recently, we showed that nanoparticles loaded with the flavonoid Hesperidin (HDN) are potential novel antimicrobial agents. N. fowleri trophozoites were treated with and without HDNconjugated with silver nanoparticles (AgNPs) and silver only, and then, 50% minimum inhibitory concentration (MIC) was determined. The results revealed that the MIC of HDN-conjugated AgNPs was 12.5 microg/mL when treated for 3 h. As no reference genome exists for N. fowleri, de novo RNA transcriptome analysis using RNA-Seq and differential gene expression analysis was performed using the Trinity software. Analysis revealed that more than 2000 genes were differentially expressed in response to N. fowleri treatment with HDN-conjugated AgNPs. Some of the genes were linked to oxidative stress response, DNA repair, cell division, cell signalling and protein synthesis. The downregulated genes were linked with processes such as protein modification, synthesis of aromatic amino acids, when compared with untreated N. fowleri. Further transcriptome studies will lead to understanding of genetic mechanisms of the biology and pathogenesis and/or the identification of much needed drug candidates. Keywords Naegleria fowleri . Transcriptome analysis . RNA-Seq
Introduction Ruqaiyyah Siddiqui and Kavitha Rajendran contributed equally to this work. Section Editor: Julia Walochnik * Naveed Ahmed Khan [email protected] 1
Department of Biology, Chemistry and Environmental Sciences, College of Arts and Sciences, American University of Sharjah, University City, Sharjah, United Arab Emirates
2
Department of Biological Sciences, School of Science and Technology, Sunway University, Bandar Sunway, Malaysia
3
Faculty of Aquatic and Fisheries Sciences, Kafrelsheikh University, Kafrelsheikh, Egypt
4
Monash University Malaysia Genomics Facility, School of Science, 47500 Bandar Sunway, Selangor Darul Ehsan, Malaysia
5
Tropical Medicine and Biology Multidisciplinary Platform, Monash University Malaysia, 47500 Bandar Sunway, Selangor Darul Ehsan, Malaysia
Naegleria fowleri are known to cause a fatal brain infection known as primary amoebic meningoencephalitis (PAM) (Maciver et al. 2020). Although infections due to these brain
Data Loading...
