Non-invasive measurement of transparency, arcus senilis, and scleral rim diameter of corneas during eye banking
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ORIGINAL PAPER
Non-invasive measurement of transparency, arcus senilis, and scleral rim diameter of corneas during eye banking Sophie Acquart • Nelly Campolmi • Zhiguo He • Giacomo Pataia • Re´my Jullienne • Olivier Garraud • Fre´de´ric Nguyen • Michel Pe´oc’h Thierry Le´pine • Gilles Thuret • Philippe Gain
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Received: 15 August 2013 / Accepted: 24 November 2013 Ó Springer Science+Business Media Dordrecht 2013
Abstract We developed a non-invasive device to quantify transparency (T), clear corneal diameter (CCD) excluding arcus senilis, and scleral rim diameter (SRD) of stored corneas. The T value (expressed in % on a relative scale), based on the modulation transfer function principle, referred to the ratio of local contrasts of a special LED backlit chart measured with and without cornea. CCD and SRD (in mm) were automatically calculated by morphologic operations. Firstly, we assessed measurement reproducibility. We then determined the agreement of T and CCD values with 3-level scores given independently by three experts on 179 scientific corneas. Thirdly, an eye bank was equipped with the device, and 358 consecutive organ-cultured (OC) corneas were tested for donor- and storage- related
factors possibly influencing T and CCD. Reproducibility of T, CCD and SRD measurements was high, with intraclass correlation coefficients of 0.982, 0.886, and 0.999 respectively. Capacity to discriminate the three levels of transparency and arcus senilis was good, with T of 20.0 (10.0–33.6), 38.3 (24.3–75.4) and 57.9 (33.9–90.0) % respectively for T deemed poor, average, and good (P \ 0.001), and CCD of 9.8 (7.3–10.6), 10.5 (8.2–11.5), and 11.1 (9.9–12.0) mm respectively for arcus senilis deemed prominent, moderate or absent (P \ 0.001). T was correlated with neither donor age nor endothelial cell density nor storage time, but slightly worsened during OC for corneas assessed twice. In conclusion, the device, which can be easily integrated in the facilities of an eye bank, provides reliable objective
S. Acquart O. Garraud Eye Bank of Saint-Etienne, Auvergne Loire Blood Center, Saint-E´tienne, France
M. Pe´oc’h Pathology Department, University Hospital, Saint-E´tienne, France
S. Acquart N. Campolmi Z. He G. Pataia R. Jullienne M. Pe´oc’h G. Thuret (&) P. Gain Corneal Graft Biology, Engineering and Imaging Laboratory, EA 2521, SFR143, Faculty of Medicine, Federative Institute of Research in Sciences and Health Engineering, Jean Monnet University, 15, Rue Ambroise Pare´, 42023 Saint-E´tienne Cedex 2, France e-mail: [email protected]
T. Le´pine Institut d’Optique Graduate School, Pole Optique Rhoˆne Alpes, Saint-E´tienne, France G. Thuret Institut Universitaire de France, Boulevard Saint-Michel, Paris, France
N. Campolmi R. Jullienne F. Nguyen G. Thuret P. Gain Ophthalmology Department, University Hospital, Saint-E´tienne, France
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Cell Tissue Bank
measurement of T, CCD, and SRD. This could be a useful tool for standardizing quality assessment of stored corneas and consequently opt
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