Optimization and Characterization of PHA (SCL-SCL) Copolymer by Indigenous Bacillus thuringiensis A102 Strain for Biomed
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Optimization and Characterization of PHA (SCL‑SCL) Copolymer by Indigenous Bacillus thuringiensis A102 Strain for Biomedical Applications Ponnusamy P. Suguna1 · Viswanathan V. Saranya1 · Periasamy P. Abirami2 · Rajaiah R. Shenbagarathai3,4 Received: 3 October 2019 / Accepted: 22 September 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract PHA is one of the leading commercially important bio-polyesteric compounds piled up as an intracellular lipid-based energy storage compound by numerous microorganisms. An indigenous Gram-positive bacterium isolated from fire ant (Solenopsis invicta) has known to potentially accumulate PHA. Various nutritional elements like carbon, nitrogen, phosphate and C/N ratio were optimized. The indigenous B.t.A102 strain grown in optimized RC medium yielded PHA of about 3.25 g/L. The extracted polymer was characterized by NMR, GC–MS, X-ray diffraction and thermal analysis via TGA & DTA. The characterized PHA was used to prepare scaffold using the solvent casting method. The non-toxic nature of the composite material was evaluated on NIH/3T3 fibroblast cell lines using different staining (like Giemsa staining, AO/EB dual staining, neutral red staining) techniques and cell viability assay. This paper dealt with the optimization of the media components that increase PHA production and primary in vitro testing for its possible application as wound dressing materials.
Introduction Plastics are considered as one of the greatest inventions of modern age. The high durability and non-degradability of synthetic polymers such as polyethylene, polypropylene, polystyrene, poly (vinyl chloride) and poly (ethylene terephthalate) causing a major problem with respect to land filling. Burning of plastic waste releases various poisonous compounds such as polycyclic aromatic hydrocarbons (PAHs), polychlorinated dibenzofurans (PCDFs) and dioxins Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00284-020-02221-x) contains supplementary material, which is available to authorized users. * Ponnusamy P. Suguna [email protected] * Rajaiah R. Shenbagarathai [email protected] 1
DBT‑BIF Centre, Department of Biotechnology, Lady Doak College, Madurai, India
2
Department of Biotechnology, Lady Doak College, Madurai, India
3
PG and Research Department of Zoology, Lady Doak College, Madurai, India
4
N. Rama Varier Ayurveda Foundation, Madurai, India
into the environment. These toxic compounds cause various respiratory illness, chronic nervous disorders, lung disease and cancer in human beings [1]. But microbial polyesters such as polyhydroxyalkanoates (PHAs), xanthan, curdlan, pullulan, hyaluronic acid are being produced by a wide range of microorganisms and completely metabolized into harmless, naturally occurring molecules. PHAs are one amongst the significant group of microbial polyesters that have been intensively examined and commercialized due to unique combination of features including its elasticity, bio
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