Prevention of Renal Scarring in Acute Pyelonephritis by Probiotic Therapy: an Experimental Study
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Prevention of Renal Scarring in Acute Pyelonephritis by Probiotic Therapy: an Experimental Study Nastaran Sabetkish 1 & Shabnam Sabetkish 1 & Mohammad Javad Mohseni 1 & Abdol-Mohammad Kajbafzadeh 1
# Springer Science+Business Media, LLC, part of Springer Nature 2017
Abstract We evaluated the protective effects of probiotic administration as a prophylaxis treatment and immediately after fever onset in increasing the immune response and decreasing the renal scarring in a rat model of acute pyelonephritis. Twenty-four rats were apportioned to three groups. In GI (n = 8), the rats were injected with direct inoculation of Escherichia coli into the right kidney. In GII (n = 8), the rats received a probiotic regimen 1 month before E. coli injection and the probiotic regimen was continued for the next 2 months. In GIII (n = 8), the probiotic regimen was started just after E. coli injection and was continued for 2 months. Technetium-99m-DMSA renal scan, histopathological evaluations, concentrations of CA19-9, IgA, blood urea nitrogen (BUN), and creatinine were assessed 1 and 2 months post-injection. It took an average of 4.2 ± 1.1 h between the injection and onset of fever in GI and GII. In GIII, this period was longer (7.5 ± 1.4). Probiotic administration resulted in reduction of interstitial fibrosis and tubular and glomerular atrophy in GII in all follow-ups. Technetium-99m-DMSA renal scan showed that the right kidney reached near the normal cortical integrity (47%) in GII compared to GI (32%) after 2 months of injection. However, the renal integrity did not improve significantly in GIII (41%). In GII, CA19-9 was lower (p < 0.05), while the levels of serum and fecal IgA were higher (p < 0.05). Administration of the probiotic regimen in the rat model may decrease renal damage in pyelonephritis. In spite of better results in the prophylactic group compared to the treatment group, no strong evidence was found to prove the advantage of its prophylactic application over the treatment administration. Keywords Acute pyelonephritis . Fever . Probiotics
Abbreviations APN UTI E. coli IgA BUN
Acute pyelonephritis Urinary tract infection Escherichia coli Immunoglobulin A Blood urea nitrogen
Introduction End-stage renal failure occurs in approximately 8–20% of children with acute pyelonephritis (APN) and young adults [1]. APN presents with nonspecific manifestations while * Abdol-Mohammad Kajbafzadeh [email protected] 1
Pediatric Urology and Regenerative Medicine Research Center, Section of Tissue Engineering and Stem Cells Therapy, Children’s Hospital Medical Center, Tehran University of Medical Sciences, No. 62, Dr. Qarib’s Street, Keshavarz Boulevard, Tehran 1419433151, Iran
specific signs usually commence after a gap of approximately 1–3 days from the initiation of infection. However, postponed diagnosis and management of APN may result in irreversible renal scar formation [2]. It has been documented that the first febrile urinary tract infection (UTI) could result in renal scarring in 5 to 15% of children [3]. Imme
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