Proteomic Exploration of Listeria monocytogenes for the Purpose of Vaccine Designing Using a Reverse Vaccinology Approac
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Proteomic Exploration of Listeria monocytogenes for the Purpose of Vaccine Designing Using a Reverse Vaccinology Approach Shivani Srivastava1 · Suraj Kumar Sharma1 · Vivek Srivastava1 · Ajay Kumar1 Accepted: 22 October 2020 © Springer Nature B.V. 2020
Abstract Listeriosis is a major foodborne infection provoked by a bacterium known as Listeria monocytogenes. It is one of the predominant causes of death in pregnant women, infants, and immunocompromised persons. Despite such fatal effects, until now there is no proper medication or drug available for such a serious foodborne infection. One of the most promising ways to deal with this challenge is vaccination. This present study aims at the prediction of B cell epitopes for subunit vaccine designing against Listeria monocytogenes using a reverse vaccinology approach. Among screened out 299 epitopes of strain F2365 of Listeria monocytogenes, based on the VaxiJen score, the top 20 epitopes were selected. 3D modeling of epitopes and alleles was generated by PEPstrMOD and Swiss Model respectively. Molecular docking reveals 4 epitopes viz., MKFLFPLKL, CEETFGIRL, FLKIDPPIL, and VRHHGGGHK based on binding energy. All 4 epitopes were investigated for non-toxicity, binding affinity, and population coverage. After vigorous investigation, epitope FLKIDPPIL was anticipated as the best vaccine contender. The stability of the FLKIDPPIL-HLA DRB1 _0101 complex was proved by performing the simulation. Here, predicted peptide through the Insilico approach may become a potential remedy against listeriosis, after the wet-lab approach and clinical trials. Keywords Listeriosis · B cell epitopes · Docking · Simulation · Reverse vaccinology
Introduction Changing food habits, advancement in technology regarding the preservation of food products for a longer time, and the ability of microorganisms to grow in adverse conditions are leading to the emergence of the foodborne infection, known as Listeriosis. The genus Listeria consists of seventeen species. Only the three hemolytic species viz., Listeria monocytogenes, Listeria seeligeri, and Listeria ivanovii are considered pathogens. Of these, Listeria monocytogenes is consistently pathogenic and is involved in foodborne outbreaks of listeriosis (Abdelhamed et al. 2019). Based on Gram-staining, Listeria monocytogenes comes under the category of Gram-positive. It shows extreme resistance in conditions like very high temperatures or very low temperatures. These bacteria have a rod-like shape and can form * Ajay Kumar [email protected] 1
Department of Biotechnology, Faculty of Engineering and Technology, Rama University Uttar Pradesh, Kanpur 209217, India
small chains (Sallami et al. 2006). Listeria monocytogenes mainly affects women who are pregnant, infants, elders above 65 years of age, and immunocompromised people (CDC 2019). Foodborne infection in humans occurs through the consumption of contaminated foods, particularly unpasteurized milk, soft cheeses, vegetables, and prepared meat products. Listeria monocytogenes show completely di
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