Protoplast Cultures
Protoplasts are used to investigate a broad range of physiological problems reaching from the significance of the cell wall for nutrient uptake to mechanisms related to the synthesis of the cell wall. This chapter described methods to obtain protoplasts a
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lant Cell and Tissue Culture – A Tool in Biotechnology Basics and Application Second Edition
Plant Cell and Tissue Culture – A Tool in Biotechnology
Karl-Hermann Neumann • Ashwani Kumar • Jafargholi Imani
Plant Cell and Tissue Culture – A Tool in Biotechnology Basics and Application Second Edition
Karl-Hermann Neumann (deceased) Hungen, Germany
Ashwani Kumar Department of Botany University of Rajasthan Jaipur, India
Jafargholi Imani Institute of Phytopathology Justus-Liebig University Giessen Giessen, Germany
ISBN 978-3-030-49096-6 ISBN 978-3-030-49098-0 https://doi.org/10.1007/978-3-030-49098-0
(eBook)
# Springer Nature Switzerland AG 2009, 2020 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors, and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, expressed or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. This Springer imprint is published by the registered company Springer Nature Switzerland AG. The registered company address is: Gewerbestrasse 11, 6330 Cham, Switzerland
Preface to First Edition
This book is intended to provide a general introduction to this exciting field of plant cell and tissue culture as tool in biotechnology, without overly dwelling on detailed descriptions of all aspects. It is aimed at the newcomer, but will hopefully also stimulate some new ideas for the “old hands” in tissue culture. Nowadays, with the vast amount of information readily available on the internet, our aim was rather to distill and highlight overall trends, deeming that a complete report of each and every tissue culture investigation and publication was neither possible, nor desirable. For some techniques, however, detailed protocols are given. We have tried to be as thorough as possible, and regret if we have inadvertently overlooked any pertinent literature or specific development that belong in this work. The three authors have been associated for many years, and have worked together on various aspects in this field. Without this close interaction, this book would not
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