Regulatory Effects of Cytokine Production in Atopic Allergic Reaction by Gammi-Danguieumja
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Regulatory Effects of Cytokine Production in Atopic Allergic Reaction by Gammi-Danguieumja Ho-Jeong Na,1 Hye-Young Shin,1 Na-Hyung Kim,1,2 Mi-Won Kwon,3 Eun-Jeong Park,3 Seung-Heon Hong,2 Nam-Il Kim,1 and Hyung-Min Kim1,4
Abstract—Gammi-danguieumja (GD) is clinically used in South Korea for treating atopic dermatitis. However, its effects in experimental models remain unknown. We investigated a possible effect of GD on cytokines production using human T cell line (MOLT-4) or human mast cell line. As a result, GD (0.01 mg/mL)-containing medium in stimulated culture supernatants increased IL-2 and IFN-γ , and decreased IL-4 secretion in MOLT-4. GD (0.01–1 mg/mL)-containing medium in stimulated culture supernatants dose-dependently and significantly decreased IL-8, IL-13, and tumor necrosis factor-alpha secretion on the phorbol 12-myristate 13-acetate and A23187-stimulated HMC-1. In addition, GD inhibited histamine release from activated mast cells. These results suggest that GD contributes to the regulation of atopic allergic reactions. KEY WORDS: Gammi-danguieumja; atopic dermatitis; cytokines; histamine.
INTRODUCTION
and anti-allergic effect (1–4). In addition, angelan, a purified polysaccharide from an Angelica gigas, showed a characteristic immunostimulation (2). Taraxacum platycarpum extract led to the isolation of a desacetylmatricarin as an active principle responsible for the anti-allergic property (5). N-(p-coumaroyl)serotonin(CS) isolated from Carthamus tinctorius inhibited the production of proinflammatory cytokines (6). The immuno regulatory mechanism of GD, remains unknown, although the clinical systemic effect in restraining the development of atopic allergic diseases is excellent. AD is a common, chronic, relapsing, inflammatory skin disorder characterized by typically distributed eczematous skin lesions with lichenification, pruritic excoriations and a susceptibility to cutaneous infections (7, 8). The prevalence of AD has steadily increased during the past few decades (9). Recent interest has focused on the role of Th-1 and Th-2 dependent cytokines in human AD (10). The Th1dependent cytokines, such as IFN-γ , IL-2, and IL-12 promote cell-mediated immunity, whereas Th2-dependent cytokines IL-4, IL-5, IL-6, and IL-13 support humoral immunity and IgE production, which is characteristic of
Gammi-danguieumja (GD) is an Oriental herbal prescription, which has been used for the treatment of atopic allergic diseases, such as atopic dermatitis (AD) and psoriasis in South Korea. The reported biological activities of the ingredients of GD include anti-allergic activities and immune functions. Recently there is growing evidence for anti-inflammatory and anti-allergic effect of the ingredients of GD. For examples, Rehmannia glutinosa, Angelica gigas, Astragalus membranceus, and Spatholobus suberectus have therapeutic action by regulating cellmediated immunity as well as anti-inflammatory activity 1 College
of Oriental Medicine, Kyung Hee University, 1 Hoegi-Dong, Dongdaemun-Gu, Seoul, 130-701, South Korea. 2
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