Shifting to a control diet after a high-fat, high-sucrose diet intake induces epigenetic changes in retroperitoneal adip
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ORIGINAL PAPER
Shifting to a control diet after a high-fat, high-sucrose diet intake induces epigenetic changes in retroperitoneal adipocytes of Wistar rats G. Uriarte & L. Paternain & F. I. Milagro & J. A. Martínez & J. Campion
Received: 14 November 2012 / Accepted: 21 December 2012 # University of Navarra 2013
Abstract The aim of the study was to analyze the phenotypic and epigenetic changes induced by the shift to a chow diet after an obesogenic environment. Animals were randomized to fed chow (control group) or high-fat–sucrose diet (HFS). After 10 weeks, half of the rats fed with HFS diet were reassigned to a chow diet (rest group) while the other half continued with the obesogenic diet (HFS group) until week 20. Changes in fat content, biochemical profile, and DNA methylation levels of several gene promoters from retroperitoneal adipocytes were analyzed. HFS diet intake for 10 weeks induced obese phenotype in the animals, increasing body weight and fat content. These effects were maintained until the end of the trial in HFS group, where an increase in liver fat content, a modification of lipid profile, and retroperitoneal adipose tissue hypertrophy were also observed. Changing the dietary pattern reversed these parameters. Epigenetic analysis showed that HFS diet intake for 20 weeks hypermethylated several CpG sites (6.7 and 29.30) and hypomethylated CpG site 15 from Electronic supplementary material The online version of this article (doi:10.1007/s13105-012-0231-6) contains supplementary material, which is available to authorized users. G. Uriarte : L. Paternain : F. I. Milagro : J. A. Martínez : J. Campion (*) Department of Nutrition, Food Science and Physiology, University of Navarra, c/ Irunlarrea 1, 31008 Pamplona, Spain e-mail: [email protected]
leptin gene promoter. Moreover, the obesogenic diet also hypomethylated CpG site 1 from Fasn (fatty acid synthase) gene promoter, without changes on Ppargc1a (peroxisome proliferator-activated receptor gamma coactivator 1-alpha), Srebf1 (sterol regulatory element-binding transcription factor 1), and aquaporin 7. Shifting to a chow diet reverted HFS-induced DNA methylation levels of some CpG sites of leptin promoter. Changing the dietary pattern hypomethylated a CpG site of Srebf1 and hypermethylated other CpGs on Ppargc1a and Fasn promoter. This study shed light on the reversibility of phenotypical and epigenetic changes induced by a HFS diet intake. Keywords Dietary pattern . Adipose . MALDI-TOF . DNA methylation
Introduction Obesity has been described as a clinical condition that may have adverse effects on health, leading to reduced life expectancy and/or increased health complications [11], and it has become one of the most serious health problems of the modern society [40]. The excessive accumulation of adipose tissue that characterises obesity is considered an important risk factor for the development of diseases such as type 2 diabetes and cardiovascular disease [14]. The rise in the prevalence of obesity appears to be the result of changes in food in
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