Structure Function Correlation on Rat Kidney Quantitative Correlatio
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Vol. 70
Editors W. Hild, Galveston J. van Limborgh, Amsterdam" R. Ortmann, KOln J.E. Pauly, Little Rock T.H. Schiebler, Wiirzburg
Walter Pfaller
Structure Function Correlation on Rat Kidney Quantitative Correlation of Structure and Function in the N onnal and Injured Rat Kidney
With 23 Figures
Springer-Verlag Berlin Heidelberg New York 1982
Dr. Walter Pfaller Institut ftir Physiologie und Balneologie der UniversWit Innsbruck Fritz-Pregl-Stra£e 3 A-601O Innsbruck Osterreich
e- ISBN-13: 978-3-642-68287-2 ISBN-13: 978-3-540-11074-3 DOl: 10.1007/978-3-642-68287-2
This work is subject to copyright. All rights are reserved, whether the whole or part of the material is concerned, specifically those of translation, reprinting, re-use of illustrations, broadcasting, reproduction by photocopying machine or similar means, and storage in data banks. Under § 54 of the German Copyright Law where copies are made for other than private use a fee is payable to "Verwertungsgesellschaft Wort", Munich. © Springer Verlag Berlin Heidelberg 1982
The use of general descriptive names, trade names, trade marks, etc. in this publication, even if the former are not especially identified, is not to be taken as a sign that such names, as understood by the Trade Marks and Merchandise Marks Act, may accordingly be used freely by anyone. Composition: Schreibsatz Service Weihrauch, Wiirzburg 2121/3321-543210
Contents
1
Introduction ...... . . . . . . . . . . . . . ..... .
2 2.1 2.2 2.3 2.4 2.4.1 2.4.2 2.4.3 2.4.4
Organization of the Rat Kidney ............ Cortex . . . . . . . . . . . . . . . . . . . . . . . . . . . .. Outer Zone of Renal Medulla . . . . . . . . . . . . .. Inner Zone of Renal Medulla . . . . . . . . . . . . . . Nephron Structure .. . . . . . . . . . . . . . . . . . .. Proximal Tubule . . . . . . . . . . . . . . . . . . . . . . Thin Limb of Henle's Loop . . . . . . . . . . . . . . . Distal Nephron. . . . . . . . . . . . . . . . . . . . . . .. Collecting Duct .. . . . . . . . . . . . . . . . . . . . ..
3 3.1 3.2 3.3 3.4
Basic Principles of Stereology . . . . . . . . . . . . .. 6 Structural Parameters and Profiles on Sections . .. 7 Methods of Estimating Volume Densities ...... 7 Estimation of Surfaces. . . . . . . . . . . . . . . . . .. 8 Estimation of Length . . . . . . . . . . . . . . . . . . . 11
4 4.1 4.1.1 4.1.2
2 3 3 5 5 5 5 5 6
Material and Methods . . . . . . . . . . . . . . . . . . . Stereological Analysis . . . . . . . . . . . . . . . . . .. Animal Preparation . . . . . . . . . . . . . . . . . . . .. Sampling of the Normal Rat Kidney for Stereology. . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. 4.1.3 Specimen Preparation ..... . . . . . . . . . . . . .. Stereological Analysis . . . . . . . . . . . . . . . . . .. 4.1.4 4.1.4.1 Light Optimal Level (Cortex and Outer Stripe of Outer Medulla) . . . . . . . . . . . . . . . . . . . . . . .. 4.1.4.2 Low Electron Microscopic Magnification. . . . . .. 4.1.5 Stereological Analysis of the Injured Rat Kidney .
13 14 14
4.2 4.3 4.3.1 4.3.2 4.3.3
Dete
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