The Application of Decellularized Adipose Tissue Promotes Wound Healing
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ORIGINAL ARTICLE
The Application of Decellularized Adipose Tissue Promotes Wound Healing Zenan Xia1 • Xiao Guo1 • Nanze Yu1 • Ang Zeng1 • Loubin Si1 • Fei Long1 Wenchao Zhang1 • Xiaojun Wang1 • Lin Zhu1 • Zhifei Liu1
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Received: 14 May 2020 / Revised: 7 July 2020 / Accepted: 20 July 2020 Ó The Korean Tissue Engineering and Regenerative Medicine Society 2020
Abstract BACKGROUND: Due to adipose-derived stem cells (ASCs) being easy to obtain, their rapid proliferation rate, and their multidirectional differentiation capabilities, they have been widely used in the field of regenerative medicine. With the progress of decellularized adipose tissue (DAT) and adipose tissue engineering research, the role of DAT in promoting angiogenesis has gradually been emphasized. METHODS: We examined the biological characteristics and biosafety of DAT and evaluated the stem cell maintenance ability and promotion of growth factor secretion through conducting in vitro and in vivo studies. RESULTS The tested ASCs showed high rat:es of proliferation and adhered well to DAT. The expression levels of essential genes for cell stem maintenance, including OCT4, SOX2, and Nanog were low at 2–24 h and much higher at 48 and 96 h. The Adipogenic expression level of markers for ASCs proliferation including PPARc, C/EPBa, and LPL increased from 2 to 96 h. Co-culture of ASCs and DAT increased the secretion of local growth factors, such as VEGF, PDGF-bb, bFGF, HGF, EGF, and FDGF-bb, and secretion gradually increased from 0 to 48 h. A model of full-thickness skin defects on the back of nude mice was established, and the co-culture of ASCs and DAT showed the best in vivo treatment effect. CONCLUSION: The application of DAT promotes wound healing, and DAT combined with ASCs may be a promising material in adipose tissue engineering and regenerative medicine. Keywords Decellularized adipose tissue Adipose-derived stem cells Nude mouse model Wound healing
1 Introduction
Zenan Xia and Xiao Guo have contributed equally to this study. Lin Zhu and Zhifei Liu share the corresponding relationship equally. & Lin Zhu [email protected] & Zhifei Liu [email protected] 1
Department of Plastic Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Shuaifuyuan 1#, Dongcheng District, Beijing 100730, China
Incurable wounds and large-scale skin defects are often caused by trauma, burns, tumor resection, radiation exposure, and certain systemic diseases [1]. Nearly half of these nonhealing chronic wounds do not respond sufficiently to existing treatments [2]. Clinically used repair methods, such as skin flaps and flap transplants, require sacrificing the donor site, causing significant damage and have a weak curative effect. Research and development of stem cells provide new ideas for solving this problem [2]. In 2001, Zuk et al. [3] isolated adipose-derived stem cells (ASCs) for the first time. Due to ASCs being easy to obtain, proliferating rapidly, and having multidirectional
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Tissue Eng Rege
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