The construction and application of a new 17-plex Y-STR system using universal fluorescent PCR
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ORIGINAL ARTICLE
The construction and application of a new 17-plex Y-STR system using universal fluorescent PCR Jinding Liu 1 & Rongshuai Wang 2 & Jie Shi 1 & Xiaojuan Cheng 1 & Ting Hao 1 & Jiangling Guo 1 & Jiaqi Wang 1 & Zidong Liu 1 & Wenyan Li 1 & Haoliang Fan 3 & Keming Yun 1 & Jiangwei Yan 1 & Gengqian Zhang 1 Received: 19 February 2020 / Accepted: 30 March 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Y-chromosomal short tandem repeat (Y-STR) polymorphisms are useful in forensic identification, population genetics, and human structures. However, the current Y-STR systems are limited in discriminating distant relatives in a family with a low discrimination power. Increasing the capacity of detecting Y chromosomal polymorphisms will drastically narrow down the matching number of genealogy populations or pedigrees. In this study, we developed a system containing 17 Y-STRs that are complementary to the current commercially available Y-STR kits. This system was constructed by multiplex PCR with expected sizes of 126–400 bp labeled by different fluorescence molecules (DYS715, DYS709, DYS716, DYS713, and DYS607 labeled by FAM; DYS718, DYS723, DYS708, and DYS714 labeled by JOE; DYS712, DYS717, DYS721, and DYS605 labeled by TAMRA; and DYS719, DYS726, DYS598, and DYS722 labeled by ROX). The system was extensively tested for sensitivity, male specificity, species specificity, mixture, population genetics, and mutation rates following the Scientific Working Group on DNA Analysis Methods (SWGDAM) guidelines. The genetic data were obtained from eight populations with a total of 1260 individuals. Our results showed that all the 17 Y-STRs are human- and male-specific and include only one copy of the Ychromosome. The 17 Y-STR system detects 143 alleles and has a high discrimination power (0.996031746). Mutation rates were different among the 17 Y-STRs, ranging from 0.30 to 3.03%. In conclusion, our study provides a robust, sensitive, and costeffective genotyping method for human identification, which will be beneficial for narrowing the search scope when applied to genealogy searching with the Y-STR DNA databank. Keywords Y-STRs . Chinese population . Forensic DNA . Mutation rate . Human identification
Introduction Jinding Liu and Rongshuai Wang contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00414-020-02291-3) contains supplementary material, which is available to authorized users. * Keming Yun [email protected] * Jiangwei Yan [email protected] * Gengqian Zhang [email protected] 1
School of Forensic Medicine, Shanxi Medical University, Jinzhong 030619, Shanxi, China
2
Chongxin Judicial Expertise Center, Wuhan 430030, Hubei, China
3
School of Basic Medicine and Life Science, Hainan Medical University, Haikou 571199, Hainan, China
With the rapid development of DNA analysis technology, STR genotyping methods consisting of multiplex PCR with fluorescently labeled primers and capillary electrophores
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