Transferrin recognition based on a protein imprinted material prepared by hierarchical imprinting technique
- PDF / 377,791 Bytes
- 8 Pages / 595.276 x 790.866 pts Page_size
- 27 Downloads / 170 Views
ORIGINAL PAPER
Transferrin recognition based on a protein imprinted material prepared by hierarchical imprinting technique Qinran Li & Kaiguang Yang & Jinxiang Liu & Lihua Zhang & Zhen Liang & Yukui Zhang
Received: 22 October 2012 / Accepted: 8 April 2013 # Springer-Verlag Wien 2013
Abstract A novel kind of transferrin imprinted polymer particles was synthesized by a hierarchical strategy. First, transferrin was immobilized on silica beads by non-covalent absorption. Then, a pre-polymerization mixture, composed of 1,4-bis(acryloyl)piperazine, methacrylamide, methacrylic acid, ammonium sulfate and polyoxyethylene sorbitan monolaurate, was irrigated into the pores of silica particles, and polymerized at 25 °C. Finally, the silica matrix was etched with ammonium hydrogen fluoride, not only to remove the template protein, but also to expose protein recognition sites on the surface of the imprinted polymer. The binding capacity of the transferrin-imprinted particles is 6.3 mg of protein per gram of material, and the time required to reach adsorption equilibrium was less than 10 min. The imprinting factor of transferrin is ca. 3.3 in the presence of ribonuclease B, cytochrome c and β-lactoglobulin. The results indicate that these imprinted polymer particles can recognize transferrin with good selectivity, high binding capacity and fast mass transfer. They may be applied as an artificial antibody to remove the high abundance proteins in plasma. Keywords Hierarchical imprinting . Protein imprinted polymers . Transferrin . Selective recognition
Q. Li : K. Yang : J. Liu : L. Zhang (*) : Z. Liang : Y. Zhang National Chromatographic R.&.A Center, Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China e-mail: [email protected] Q. Li : J. Liu University of Chinese Academy of Sciences, Beijing 100039, China
Introduction Proteomics research, with aims to identify proteins in a cell, tissue, or body fluid to investigate various biological problems, is of great significance [1, 2]. Serum, as one of the most popular proteomic research targets, contains a dynamic concentration range of protein components. Among them, several groups of high-abundance proteins, such as human serum albumin (HSA), immunoglobulins G (IgG), transferrin, and haptoglobin, account for 90 % of the total protein mass, and seriously interfere with the detection of lowabundance potential biomarkers [3, 4]. As a result, the selective removal of high abundant proteins in biological sample is beneficial to identify low-abundant ones. Transferrin, with the molecular weigh of about 77 kDa, was the third highest abundant proteins in plasma [5, 6]. At present, the antibody columns for HSA or IgG was widely available, but not for transferrin. Therefore, it was necessary to fabricate protein recognized material towards transferrin with high selectivity, which might become an artificial antibody to remove transferrin from plasma. Molecularly imprinting technology is an a
Data Loading...
