Quantitative trait locus mapping reveals conservation of major and minor loci for powdery mildew resistance in four sour
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Quantitative trait locus mapping reveals conservation of major and minor loci for powdery mildew resistance in four sources of resistance in mungbean [Vigna radiata (L.) Wilczek] Sompong Chankaew • Prakit Somta • Takehisa Isemura • Norihiko Tomooka • Akito Kaga • Duncan A. Vaughan • Peerasak Srinives Received: 5 September 2012 / Accepted: 7 March 2013 / Published online: 3 April 2013 Ó Springer Science+Business Media Dordrecht 2013
Abstract Powdery mildew (PM) is a common and serious disease of mungbean [Vigna radiata (L.) Wilczek]. A few quantitative trait loci (QTL) for PM resistance in mungbean have been reported. The objective of this study was to locate QTL for PM resistance in two resistant accessions V4718 and RUM5. Simple sequence repeat markers were analyzed in an F2 population from a cross between Kamphaeng Saen 1 (KPS1; susceptible to PM) and V4718 (resistant to PM), and in F2 and BC1F1 populations from a cross between Chai Nat 60 (CN60; susceptible to PM) and RUM5 (resistant to PM). Progenies of 134 F2:3 and F2:4 lines derived from KPS1 9 V4718, and 190 F2:3 and 74 BC1F1:2 lines
Electronic supplementary material The online version of this article (doi:10.1007/s11032-013-9856-6) contains supplementary material, which is available to authorized users.
derived from CN60 9 RUM5 and CN60 9 (CN60 9 RUM5), respectively, were evaluated for response to PM under field conditions. Multiple interval mapping identified a major QTL on linkage group (LG) 9 and two minor QTL on LG4 for the resistance in V4718, and detected two major QTL on LG6 and LG9 and one minor QTL on LG4 for the resistance in RUM5. Comparative linkage analysis of the QTL for PM resistance in this study and in previous reports suggests that the resistance QTL on LG9 in V4718, RUM5, ATF3640 and VC6468-11-1A are the same locus or linked. One QTL on LG4 is the same in three sources (V4718, RUM5 and VC1210A). Another QTL on LG6 is the same in two sources (RUM5 and VC6468-11-1A). In addition, one QTL in V4718 on LG4 appears to be a new resistance locus. These different resistance loci will be useful for breeding durably PM-resistant mungbean cultivars.
S. Chankaew Program in Plant Breeding, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Kamphaeng Saen, Nakhon Pathom 73140, Thailand
Keywords Mungbean Powdery mildew Erysiphe polygoni SSR Vigna radiata QTL Host–plant resistance
P. Somta P. Srinives (&) Department of Agronomy, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Kamphaeng Saen, Nakhon Pathom 73140, Thailand e-mail: [email protected] T. Isemura N. Tomooka A. Kaga D. A. Vaughan Genetic Resources Center, National Institute of Agrobiological Sciences, Kannondai 2-1-2, Tsukuba, Ibaraki 305-8602, Japan
Introduction Mungbean [Vigna radiata (L.) Wilczek] is an important legume of South and Southeast Asia, where 90 % of the world’s mungbean is produced annually. Mungbean seeds contain 20–25 % protein and 60–65 % carbohydrate (Lambrides and Godwin
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2007). It is a major inexpensive source
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