Mapping quantitative trait loci conferring blast resistance in upland indica rice ( Oryza sativa L.)
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J. Crop Sci. Biotech. 2011 (March) 14 (1) : 57 ~ 63 DOI No. 10.1007/s12892-010-0030-y RESEARCH ARTICLE
Mapping Quantitative Trait Loci Conferring Blast Resistance in Upland Indica Rice (Oryza sativa L.) Puji Lestari1*, Kurniawan Rudi Trijatmiko1,2, Reflinur1, Ahmad Warsun1, Tasliah1, Isabelita Ona2, Casiana Vera Cruz2, Masdiar Bustamam1*
Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development, Jl. Tentara Pelajar No.3A Cimanggu, Bogor 16111, Indonesia 2 International Rice Research Institute, College, Los Banos, Laguna 4031, Philippines Egually contributed 1
Received: February 16, 2010 / Revised: March 24, 2010 / Accepted: December 15, 2010 Ⓒ Korean Society of Crop Science and Springer 2011
Abstract A genetic analysis of blast resistance in upland rice variety is very crucial. In this study, we performed a linkage mapping of quantitative trait loci (QTLs) for blast resistance using an advanced backcross population from a cross between Way Rarem (susceptible indica variety) and Oryzica Llanos 5 (durable resistant indica variety). A transgressive segregation was observed in the advanced backcross population of Way Rarem//Oryzica Llanos 5. A total of 16 QTLs have been identified along chromosomes 1, 3, 5, 6, 7, 8, 9, and 11 against eight blast pathogen isolates. Each QTL accounted from 11.31 to 45.11% of the variation in blast resistance. Most QTLs showed race specificity, demonstrating the small effect of such QTLs. Unexpectedly, several superior blast resistance alleles were contributed by Way Rarem, the susceptible-recurrent parent. Among eight candidate defense response genes detected in several loci, a single gene (oxalate oxidase) present on chromosome 3 was found to be associated with blast resistance in upland indica rice. Ultimately, these advanced backcross lines with resistance to blast tagged by markers might be useful for pyramiding blast resistance alleles in upland rice. Key words: blast resistance, Oryza sativa, QTL analysis, upland rice
Introduction The rice blast caused by the fungus Pyricularia oryzae B. Couch (anomorph Pyricularia oryzae Cavara), which, in its sexual state, is known as Magnaporthe oryzae (Couch and Kohn 2002), is one of the most destructive diseases and major constraint of rice production worldwide. Blast was first reported in Asia more than three centuries ago and is now present in over 85 countries, including in the upland rice ecosystem in Indonesia (Rao 1994). A great deal of upland rice varieties show greater resistance to blast compared to lowland varieties. The infection Masdiar Bustamam ( ) E-mail: [email protected] Puji Lestari ( ) E-mail: [email protected]
The Korean Society of Crop Science
of blast fungus is to be more conducive in upland conditions and sometimes causes many yield losses. Growing resistant varieties has been the most effective and economical way to control rice blast disease (Hirano 1994). Many resistant varieties have been deployed, however extensive application of resistant rice variety especially tho
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