Rapid and Simple Determination of Sarafloxacin in Egg by Time-Resolved Chemiluminescence
- PDF / 295,868 Bytes
- 9 Pages / 595.276 x 790.866 pts Page_size
- 15 Downloads / 180 Views
Rapid and Simple Determination of Sarafloxacin in Egg by Time-Resolved Chemiluminescence José Antonio Murillo Pulgarín & Aurelia Alañón Molina & Fernando Martínez Ferreras & Pablo Fernández López
Received: 27 June 2012 / Accepted: 16 October 2012 / Published online: 7 November 2012 # Springer Science+Business Media New York 2012
Abstract A new chemiluminescence method for the determination of the fluoroquinolone sarafloxacin is described. The method is based on the measurement of the chemiluminescent (CL) radiation emitted in the reaction of sarafloxacin with Ce (IV) in sulfuric acid medium and in the presence of RuðbipyÞ2þ as sensitizer. Since the 3 method allows the recording of the whole CL intensityversus-time profile, the use of measurement parameters such as the maximum CL emission is possible, which is proportional to the analyte concentration. The optimum chemical and experimental conditions for the CL emission of the reaction were researched. The calibration graph was lineal in the concentration range from 5.0 to 30.0 mgL−1 of sarafloxacin. The limit of detection, according to Clayton et al. (Anal Chem 59:2506–2514, 1987), was 1.17 mg L−1. After analyzing a series of ten solutions of 10.0 mg L−1 of sarafloxacin, the estimated average concentration was 10.30 mgL−1 with a standard deviation of 0.42 mg L−1 (confidence level, 95 %). The effect on the sarafloxacin CL signal of some common excipients (sucrose, lactose, and starch) used widely in pharmaceutical preparations was also tested, as well as that of others fluoroquinolones (namely, enrofloxacin and ciprofloxacin). Finally, the proposed method was applied to the determination of sarafloxacin in different spiked egg samples by using the standard addition methodology, obtaining excellent recoveries in all cases (close to 100 %). Keywords Chemiluminescence . Flow techniques . Eggs . Sarafloxacin J. A. Murillo Pulgarín : A. Alañón Molina (*) : F. Martínez Ferreras : P. Fernández López Department of Analytical Chemistry and Foods Technology, University of Castilla-La Mancha, 13071, Ciudad Real, Spain e-mail: [email protected]
Abbreviations CL Chemiluminescence/Chemiluminescent RuðbipyÞ2þ Tris(2,2′-bipyridyl) ruthenium (II) 3
Introduction Quinolones are a group of very important antibacterial agents that are derived from the discovery of nalidixic acid in 1962 by Lesher. This drug was found out to be very efficient in the treatment of diseases in the liver and urine caused by many types of bacteria. The later inclusion of a fluorine atom in position six of the quinolonic ring yielded the subgroup called fluoroquinolones, composed by highpotency antibacterial agents that show even higher activity than the original quinolones (Jackson et al. 1998), although their use is often questioned because of the possible development of resistance to antibiotics by human pathogens (Mandell et al. 2002). Fluoroquinolones cause the inhibition of the activity of DNA gyrase, an enzyme that controls the supercoiling of DNA (von Rosenstiel and Adam 1994). The 6-fluoro-1-(4
Data Loading...
