Rapid Determination of Organonitrogen, Organophosphorus and Carbamate Pesticides in Tea by Ultrahigh-Performance Liquid
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Rapid Determination of Organonitrogen, Organophosphorus and Carbamate Pesticides in Tea by Ultrahigh-Performance Liquid Chromatography–Tandem Mass Spectrometry (UPLC-MS/MS) Hong-Xia Zhao & Shan-Cang Zhao & Li-Gang Deng & Jiang-Sheng Mao & Chang-Ying Guo & Guo-Sheng Yang & Xiao Lu & Hassan Y. Aboul-Enein Received: 28 March 2012 / Accepted: 21 June 2012 / Published online: 17 July 2012 # Springer Science+Business Media, LLC 2012
Abstract A rapid and effective multiresidue method has been developed for the determination of 18 pesticide residues (selected from organonitrogen, organophosphorus, and carbamate pesticides) in tea by ultrahigh-performance liquid chromatography–tandem mass spectrometry. Tea samples were extracted with acetonitrile and purified by a novel multilayer solid-phase extraction cartridge—Cleanet TPT. Evaluation of the influence of the mobile phase on the ionization efficiency, resolution, and sensitivity was carried out by comparing the acetonitrile–water and methanol– H.-X. Zhao : G.-S. Yang (*) School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, People’s Republic of China e-mail: [email protected] S.-C. Zhao : L.-G. Deng : J.-S. Mao : C.-Y. Guo : X. Lu Central Laboratory of Shandong Academy of Agricultural Sciences, Jinan 250100, People’s Republic of China S.-C. Zhao : L.-G. Deng : J.-S. Mao : C.-Y. Guo : X. Lu Key Laboratory of Test Technology on Food Quality and Safety of Shandong Province, Jinan 250100, People’s Republic of China S.-C. Zhao : L.-G. Deng : J.-S. Mao : C.-Y. Guo : X. Lu (*) The Base of Public Service on Pesticide Residues of Shandong Province, Jinan 250100, People’s Republic of China e-mail: [email protected] H. Y. Aboul-Enein (*) Pharmaceutical and Medicinal Chemistry Department, Pharmaceuticals and Drug Industries Research Division, National Research Centre, Dokki, Cairo 12311, Egypt e-mail: [email protected]
water with different modifiers. The recoveries of all the pesticides varied from 70 to 110 % with a relative standard deviation of less than 15 %, and the determination coefficient for each pesticide was R2 >0.99. Matrix-matched standard calibration curve was used to reduce errors associated with matrix-induced enhancement or suppression effects. The limit of detection for all targeted pesticides ranged from 0.03 to 1.5 μg/kg. Applicability of this analytical approach was confirmed by successful determination of tea samples from different regions of China. Keywords Pesticides . UPLC-MS/MS . Multiresidue . Tea . Solid-phase extraction
Introduction Tea is one of the oldest and most widely consumed beverages in the world valued for its natural scent and healthpromoting properties, which have been demonstrated in many animal experiments and human studies (Jankun et al. 1997; Cabrera et al. 2006; Ju et al. 2007). However, tea plants are vulnerable to a great number of pests and diseases, and the tea fields are also easily invaded by several species of weeds. As a result, pesticides are widely applied to protect tea from pests, disease, and weed
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