Rat Genomics

This book provides both historical perspective and practical information to support researchers either currently involved in genomic research on rats or planning to begin such a project.  In numerous chapters, a detailed protocol is provided for rese

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G. Thomas Hayman Jennifer R. Smith Melinda R. Dwinell Mary Shimoyama Editors

Rat Genomics

METHODS

IN

MOLECULAR BIOLOGY

Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, UK

For further volumes: http://www.springer.com/series/7651

For over 35 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-bystep fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice. These hallmark features were introduced by series editor Dr. John Walker and constitute the key ingredient in each and every volume of the Methods in Molecular Biology series. Tested and trusted, comprehensive and reliable, all protocols from the series are indexed in Pub Med.

Rat Genomics Edited by

G. Thomas Hayman Department of Biomedical Engineering, Rat Genome Database, Medical College of Wisconsin, Milwaukee, WI, USA

Jennifer R. Smith Department of Biomedical Engineering, Rat Genome Database, Medical College of Wisconsin, Milwaukee, WI, USA

Melinda R. Dwinell Genomic Sciences and Precision Medicine Center, Medical College of Wisconsin, Milwaukee, WI, USA Department of Physiology, Rat Genome Database, Medical College of Wisconsin, Milwaukee, WI, USA

Mary Shimoyama Department of Biomedical Engineering, Rat Genome Database, Medical College of Wisconsin, Milwaukee, WI, USA

Editors G. Thomas Hayman Department of Biomedical Engineering Rat Genome Database Medical College of Wisconsin Milwaukee, WI, USA

Jennifer R. Smith Department of Biomedical Engineering Rat Genome Database Medical College of Wisconsin Milwaukee, WI, USA

Melinda R. Dwinell Genomic Sciences and Precision Medicine Center Medical College of Wisconsin Milwaukee, WI, USA

Mary Shimoyama Department of Biomedical Engineering Rat Genome Database Medical College of Wisconsin Milwaukee, WI, USA

Department of Physiology Rat Genome Database Medical College of Wisconsin Milwaukee, WI, USA

ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-9580-6 ISBN 978-1-4939-9581-3 (eBook) https://doi.org/10.1007/978-1-4939-9581-3 © Springer Science+Business Media, LLC, part of Springer Nature 2019 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by sim