Rat Genomics Methods and Protocols
Grown exponentially by the genomic revolution, the use of the rat as a model of choice for physiological studies continues in popularity and at a much greater depth of understanding. In Rat Genomics: Methods and Protocols, world-wide experts provide both
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Molecular Biology™
Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
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Rat Genomics Methods and Protocols
Edited by
Ignacio Anegon INSERM, UMR 643, Nantes, France
Editor Ignacio Anegon INSERM, UMR 643 Nantes, France [email protected]
ISSN 1064-3745 e-ISSN 1940-6029 ISBN 978-1-60327-388-6 e-ISBN 978-1-60327-389-3 DOI 10.1007/978-1-60327-389-3 Springer New York Dordrecht Heidelberg London Library of Congress Control Number: 2009936793 © Humana Press, a part of Springer Science+Business Media, LLC 2010 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, c/o Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. While the advice and information in this book are believed to be true and accurate at the date of going to press, neither the authors nor the editors nor the publisher can accept any legal responsibility for any errors or omissions that may be made. The publisher makes no warranty, express or implied, with respect to the material contained herein. Printed on acid-free paper Humana Press is part of Springer Science+Business Media (www.springer.com)
To: My beloved parents, Ignacio and Hani And to my wonderful and adorable family, Quica, Claudia, and Alex
“It’s never too late to be who you might have been.” George Eliot
Preface This book aims to provide practical information for researchers involved in genomic research in the rat along with a more contextual discussion about the usefulness of the rat in physiological or translational research in different organs and systems. The rat has been a model of choice for physiological studies, and thus a great deal of information is already available. In addition, a large number of inbred, congenic, and transgenic rat lines have already been described. An exponential growth in rat genomics is taking place based on the recent availability of genomic tools. These include genome sequencing, quantitative trait loci mapping, and the identification of single nucleotide polymorphisms as well as the development of transgenic technologies such as nuclear cloning, lentiviral-mediated transgenesis, gene knock-down using RNA interference, gene knock-out by mutagenesis, and zinc finger nucleases plus exciting advances in the obtention of rat embryonic cell lines. All of these topics a
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