Recent Advances in Thin-Layer Chromatography
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THIN-LAYER CHROMATOGRAPHY
RecentAdvances in THIN-LAYER CHROMATOGRAPHY Edited by
F. A. A. Dallas G/axo Group Research Limited Ware, United Kingdom
H. Read
BP Research Gentre Sunbury-on- Thames, United Kingdom
R. J. Ruane
and
I. D. Wilson
IGI Pharmaceuticals Division Macclesfield, United Kingdom
Springer Science+Business Media, LLC
Library of Congress Cataloging in Publieation Data Chromatographie Soeiety International Symposium on Thin-Layer Chromatography (1987: Brighton, East Sussex) Reeent advanees in thin-Iayer ehromatography. "Proeeedings of the Chromatographie Soeiety International Symposium on ThinLayer Chromatography, held Mareh 11-12, 1987, in Brighton, United Kingdom"-CIP eopr. p. Bibliography: p. Includes index. 1. Thin layer ehromatography-Congresses. I. Dallas, F. A. A. 11. Chromatographie Soeiety. 111. Tille. QD79.C8C47 1987 543 1 08956 88-17880
ISBN 978-1-4899-2223-6 ISBN 978-1-4899-2221-2 (eBook) DOI 10.1007/978-1-4899-2221-2
Proeeedings of Ihe Chromatographie Soeiety International Symposium on Thin-Layer Chromatography, held Mareh 11-12, 1987, in Brighton, United Kingdom
© 1988 Springer Science+Business Media New York Originally published by Plenum Press, New York in 1988. Softcover reprint ofthe hardcover Ist edition 1988 All rights reserved No pari of Ihis book may be reproduced, stored in a relrieval system, or transmitted in any form or by any means, electronie, meehanieal, photocopying, mierotilming, reeording, or otherwise, wilhoul written permission trom the Publisher
PREFACE
For many years TLC has suffered from the image of being a low sensitivity, low resolution, non-quantitative teehnique, suitable for chemists, but not a tool for real ehromatographers. Whilst perhaps true in the past this attitude no longer ref1eets the eapabi1ities of modern instrumenta1ized TLC in all its many forms. This volume represents the proceedings of a meeting in Brighton in 1987 whieh formed part of a eontinuing series of one and two day events on TLC organized by the Chromatographie Soeiety either alone or, like this one, in eonjunetion with other learned bodies. These meetings are designed to keep ehromatographers up to date with the latest deve10pments and help promote a more positive image of TLC. lan Wilson November 1987
v
CONTENTS
The Chromatographic Society
xi
Contemporary Thin-layer Chromatography: An introduction IoD. Wilson INSTRUMENTATION Quantitative Methods in Thin-layer Chromatography C.F. Poole, S.K. Poole and T.A. Dean
11
Fluorescence Line-narrowing Spectroscopy: A New and Highly Selective Detection Technique for Thin-layer and Liquid Chromatography J.W. Hofstraat, C. Gooijer, U.A.Th. Brinkman and N.H. Velthorst
29
Qualitative and Quantitative Image Analysis of Fluorescence from High Performance Thi.n-layer Chromatography R.M. Belchamber, S.J. Brinkworth, H. Read and J.D.M. Roberts
37
Analytical Rotation Planar Chromatography Sz. Nyiredy, K. Dallenbach-Toelke and O. Sticher
45
Analytical and Preparative Overpressured Layer Chromatography E. Mincsovics and E. Tyiha
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