Recombinant Gene Expression
Studies related to recombinant gene expression have brought new advance such as the emergence of the “omics” technologies. While Escherichia coli, Sacharomyces cerevisiae and insect cells continue to be the dominant production platforms of recombina
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Argelia Lorence Editor
Recombinant Gene Expression Reviews and Protocols Third Edition
METHODS
IN
MOLECULAR BIOLOGY™
Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Recombinant Gene Expression Reviews and Protocols, Third Edition Edited by
Argelia Lorence Arkansas Biosciences Institute, Arkansas State University, Jonesboro, AR, USA
Editor Argelia Lorence, Ph.D. Arkansas Biosciences Institute and Department of Chemistry & Physics Arkansas State University, Jonesboro University Loop E. 504 State University, AR 72401, USA [email protected]
ISSN 1064-3745 e-ISSN 1940-6029 ISBN 978-1-61779-432-2 e-ISBN 978-1-61779-433-9 DOI 10.1007/978-1-61779-433-9 Springer New York Dordrecht Heidelberg London Library of Congress Control Number: 2011943340 © Springer Science+Business Media, LLC 2012 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, c/o Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. Printed on acid-free paper Humana Press is part of Springer Science+Business Media (www.springer.com)
Dedication To David and Noah, my two big loves
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Preface Formidable progress in the field of recombinant gene expression has taken place since 2004, the date of publication of the previous edition of this book. In particular, the emergence of the “omics” technologies has revolutionized all areas of biology. In the industrial arena, Escherichia coli, Saccharomyces cerevisiae, and insect cells continue to be the dominant production platforms of recombinant proteins. However, in the last few years plants and animals have grown in importance as viable sources of more complex proteins. This third edition of Recombinant Gene Expression is a lot more than just an update of the previous edition. Although some of the authors that contributed in 2004 were also invited to participate in this new project, this volume contains brand new protocols and topics not covered before. I am indebted to the experts in the field and their students and post-doctoral associates whose talent and experience is reflected in the outstanding quality of the chapters here included. I would like to thank Dr. Paulina Balbás, from whom I have learned a lot about managing a project like this, and two members of my laboratory, Austin Slaven and Gwendolyn Wilson who helped me edit the reference sectio
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