Record of Some Digeneans, Infecting Freshwater Fishes, Snails, and Amphibians in India, Using 28S Ribosomal DNA Marker
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RESEARCH ARTICLE
Record of Some Digeneans, Infecting Freshwater Fishes, Snails, and Amphibians in India, Using 28S Ribosomal DNA Marker Kirti Choudhary1 • Shailendra Ray1 • Nirupama Agrawal1
Received: 17 December 2018 / Revised: 18 May 2019 / Accepted: 3 July 2019 The National Academy of Sciences, India 2019
Abstract Eleven species of digenetic trematodes are recorded herein in an attempt to chart the trematode biodiversity in Kakraiya Lake of District Barabanki, U.P., infecting freshwater fishes, snails, and frogs. The species are Opisthorchis attuai and Isoparorchis hypselobagri from Wallago attu; Diplostomulum minutum from Heteropneustes fossilis; Buchephalopsis karvai from Xenentodon cancila; Cephalogonimus mukerjius, and Kaurma sp. from Pila globosa; Pseudodiplodiscoides murtyi from Bellamya bengalensis; Mesocoelium sociale from Duttaphrynus melanostictus; Diplodiscus amphichrus, Halipegus mehransis, and Halipegus barabankiensis n.sp. from Euphlyctis cyanophlyctis. Among the eight species considered as valid so far O. attuai, I. hypselobagri, M. sociale, B. karvai, P. murtyi, D. amphichrus, H. mehransis and C. mukherjius have been described well only at the adult stage, whereas the morphology of D. minutum, and Kaurma sp. at the metacercarial stage has been described, which further awaits to trace their life cycle. The present
Significance The presence of numerous parasites including a newer species, from the various vertebrate and invertebrate hosts, requires attention. Developments of some parasites within the invertebrate hosts that are the usual parasites of vertebrate hosts lead to their progenetic development, due to the absence of piscine hosts. The present study is the first molecular approach of eleven species altogether from India. & Nirupama Agrawal [email protected] Kirti Choudhary [email protected] Shailendra Ray [email protected] 1
Department of Zoology, University of Lucknow, Lucknow, UP 226007, India
results show that H. barabankiensis represents distinct entities from a molecular and morphological point of views, which leads to the description of a new species. All these trematode species were subjected to morphological, morphometric, and molecular analysis. Partial nucleotide sequences of the 28S ribosomal gene were obtained from these trematode species and deposited in the GenBank. The phylogenetic reconstruction based on the 28S rRNA gene placed the studied species within the respective families, and their validity is discussed. Keywords Digenetic trematode Metacercaria Adult 28S ribosomal DNA Phylogeny
Introduction During summers (2015–2017), the authors found several invertebrate (snails) and vertebrate hosts (fishes and frogs) in the vicinity of Kakraiya Lake of District Barabanki, U.P. It is a large shallow lake, which covers about 50 acres, having an inlet of several water bodies. It is the major attraction of fishing, and the main flow of the lake is regulated by the Sharda river canal. During May–June, when it gets dry, associated lakes and
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