Redox stimulus disulfide conjugated polyethyleneimine as a shuttle for gene transfer
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DELIVERY SYSTEMS Original Research
Redox stimulus disulfide conjugated polyethyleneimine as a shuttle for gene transfer Ihsan Ullah1 Jing Zhao1 Bin Su2 Shah Rukh3 Jintang Guo1,4 Xiang-kui Ren1,4,5 Shihai Xia6 Wencheng Zhang7 Yakai Feng1,2,5 ●
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Received: 18 March 2020 / Accepted: 27 October 2020 / Published online: 28 November 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Redox-responsive cationic polymers have gained considerable attention in gene delivery due to low cytotoxicity and spatiotemporal release of DNA into the cells. Here, we reported the synthesis of reducible disulfide conjugated polyethyleneimine (1.8 kDa) (denoted as SS-PEI) and its application to transfer pEGFP-ZNF580 plasmid (pZNF580) into EA.hy926 cell. This reducible SS-PEI polymer was prepared by one-step polycondensation reaction of low molecular weight PEI with bis-(pnitrophenyl)-3,3′-dithiodipropionate. The SS-PEI successfully condensed pZNF580 into nano-sized complexes (170 ± 1.5 nm to 255 ± 1.6 nm) with zeta potentials of 3 ± 0.4 mV to 17 ± 0.9 mV. The complexes could be triggered to release pZNF580 when exposed to the reducing environment of 5 mM dithiothreitol. Besides, the SS-PEI exhibited low cytotoxicity. In vitro transfection results showed that SS-PEI exhibited good transfection efficiency comparable to PEI25kDa. Thus, the SS-PEI could act as an reducible gene carrier with good transfection efficiency and low cytotoxicity. Graphical Abstract
These authors contributed equally: Ihsan Ullah, Jing Zhao, Bin Su Supplementary information The online version of this article (https:// doi.org/10.1007/s10856-020-06457-8) contains supplementary material, which is available to authorized users. * Yakai Feng [email protected] 5
Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Weijin Road 92, Tianjin 300072, China
Department of Clinical Research, Characteristic Medical Center of Chinese People’s Armed Police Force, 220 Chenglin Road, Tianjin 300162, China
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Department of Hepatopancreatobiliary and Splenic Medicine, Affiliated Hospital, Logistics University of People’s Armed Police Force, Chenglin Road 220, Tianjin 300162, China
Department of Chemistry, School of Science, Abdul Wali Khan University, Mardan 23200, Pakistan
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Department of Physiology and Pathophysiology, Logistics University of People’s Armed Police Force, Tianjin 300309, China
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School of Chemical Engineering and Technology, Tianjin University, Yaguan Road 135, Tianjin 300350, China
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Chemical Engineering (Tianjin), Weijin Road 92, Tianjin 300072, China
Collaborative Innovation Centre of Chemical Science and
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1 Introduction Gene therapy is a potential therapeutic tool for many diseases by supplementing missing or defective genes into the cells [1]. The gene suppressing or replacing technology would make it possible to treat different life-threatening diseases including cancer. However, the journey ahead is challengin
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