Regulation of Cued Fear Expression via Corticotropin-Releasing-Factor Neurons in the Ventral Anteromedial Thalamic Nucle
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ORIGINAL ARTICLE
Regulation of Cued Fear Expression via Corticotropin-ReleasingFactor Neurons in the Ventral Anteromedial Thalamic Nucleus Yin Lv1 • Peng Chen1 • Qing-Hong Shan1 • Xin-Ya Qin1 • Xiu-Hong Qi1 Jiang-Ning Zhou1,2
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Received: 23 October 2019 / Accepted: 15 July 2020 Ó Shanghai Institutes for Biological Sciences, CAS 2020
Abstract The ventral part of the anteromedial thalamic nucleus (AMv) is in a position to convey information to the cortico-hippocampal-amygdalar circuit involved in the processing of fear memory. Corticotropin-releasing-factor (CRF) neurons are closely associated with the regulation of stress and fear. However, few studies have focused on the role of thalamic CRF neurons in fear memory. In the present study, using a conditioned fear paradigm in CRF transgenic mice, we found that the c-Fos protein in the AMv CRF neurons was significantly increased after cued fear expression. Chemogenetic activation of AMv CRF neurons enhanced cued fear expression, whereas inhibition had the opposite effect on the cued fear response. Moreover, chemogenetic manipulation of AMv CRF neurons did not affect fear acquisition or contextual fear expression. In addition, anterograde tracing of projections revealed that AMv CRF neurons project to wide areas of the cerebral cortex and the limbic system. These results
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s12264-020-00592-6) contains supplementary material, which is available to authorized users. & Peng Chen [email protected] & Jiang-Ning Zhou [email protected] 1
Hefei National Laboratory for Physical Sciences at the Microscale, School of Life Sciences, Chinese Academy of Sciences Key Laboratory of Brain Function and Diseases, University of Science and Technology of China, Hefei 230027, China
2
Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai 200031, China
uncover a critical role of AMv CRF neurons in the regulation of conditioned fear memory. Keywords Corticotropin-releasing-factor neurons Ventral anteromedial thalamic nucleus Cued fear expression Chemogenetics
Introduction The ventral part of the anteromedial thalamic nucleus (AMv) is the ventral portion of the medial part of the anterior thalamic nuclei. The anterior thalamic nuclei are known to be involved in spatial learning and navigational memory, as well as emotional memory processing related to predatory threats [1–5]. Anatomically, the anterior thalamic nuclei are in a position to convey information to the cortico-hippocampal-amygdalar circuit, which plays a critical role in the regulation of fear-related behavior in animals [6]. Previous studies have also shown that the anterior thalamic nuclei are correlated with fear-conditioned memory. Lesions of the anterior thalamic nuclei were found to strongly impair the stabilization of contextual fear memory in rats [7], and c-Fos immunoreactivity was found to be significantly increased in the
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