Regulation of JAK/STAT signal pathway by miR-21 in the pathogenesis of juvenile idiopathic arthritis
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ORIGINAL ARTICLE
Regulation of JAK/STAT signal pathway by miR‑21 in the pathogenesis of juvenile idiopathic arthritis Hong‑Wei Li1,2 · Hua‑Song Zeng1,2 Received: 27 January 2019 / Accepted: 7 May 2019 © The Author(s) 2019
Abstract Background Overexpression of the components of the Janus kinase/signal transducer and activator of transcription (JAK/ STAT) signalling pathway is the key factor of the pathogenic mechanisms underlying systemic juvenile idiopathic arthritis (sJIA). The study aims to investigate the association between miR-21 and the JAK/STAT signal pathway in JIA. Methods Total RNA was extracted from peripheral blood mononuclear cells (PBMCs) in active JIA patients. The relative expressions of miR-21, STAT3 and suppressor of cytokine signalling 3 in PBMCs were measured by real-time polymerase chain reaction and their expressions were measured by western blotting and dual-luciferase reported assay. Rheumatoid arthritis fibroblast-like synovial cell (RASF) was stimulated to become to osteoclasts using macrophage colony-stimulating factor (M-CSF) and factors that can impact on their differentiation ability were identified through the transfection of LV3miR-21. The expression of STAT3/p-STAT3 was measured by western blot, and the levels of interleukin (IL)-17A, p65, matrix metalloproteinases (MMP)-3, MMP-4 and receptor activator of nuclear factor-κB after the LV3-miR-21 transfection were tested by enzyme-linked immunosorbent assay. Finally, the miR-21 targeted STAT3 gene was detected by the dualluciferase reported assay. Results The expression of miR-21 was significantly lower in JIA patients than in healthy control (P
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