Regulatory effects of chicken TRIM25 on the replication of ALV-A and the MDA5-mediated type I interferon response
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RESEARCH ARTICLE
Open Access
Regulatory effects of chicken TRIM25 on the replication of ALV‑A and the MDA5‑mediated type I interferon response Jin‑run Zhou1,2†, Jun‑hong Liu1,2†, Hong‑mei Li1,2†, Yue Zhao1,2, Ziqiang Cheng1,2, Yan‑meng Hou1,2 and Hui‑jun Guo1,2*
Abstract This study focuses on the immunoregulatory effects of chicken TRIM25 on the replication of subgroup A of avian leukosis virus (ALV-A) and the MDA5-mediated type I interferon response. The ALV-A-SDAU09C1 strain was inocu‑ lated into DF1 cells and 1-day-old SPF chickens, and the expression of TRIM25 was detected at different time points after inoculation. A recombinant overexpression plasmid containing the chicken TRIM25 gene (TRIM25-GFP) was constructed and transfected into DF1 cells to analyse the effects of the overexpression of chicken TRIM25 on the replication of ALV-A and the expression of MDA5, MAVS and IFN-β. A small interfering RNA targeting chicken TRIM25 (TRIM25-siRNA) was prepared and transfected into DF1 cells to assess the effects of the knockdown of chicken TRIM25 on the replication of ALV-A and the expression of MDA5, MAVS and IFN-β. The results showed that chicken TRIM25 was significantly upregulated at all time points both in ALV-A-infected cells and in ALV-A-infected chickens. Overex‑ pression of chicken TRIM25 in DF1 cells dramatically decreased the antigenic titres of ALV-A in the cell supernatant and upregulated the relative expression of MDA5, MAVS and IFN-β induced by ALV-A or by poly(I:C); in contrast, knockdown of chicken TRIM25 significantly increased the antigenic titres of ALV-A and downregulated the relative expression of MDA5, MAVS and IFN-β. It can be concluded that chicken TRIM25 can inhibit the replication of ALV-A and upregulate the MDA5 receptor-mediated type I interferon response in chickens. This study can help improve the understanding of the antiviral activities of chicken TRIM25 and enrich the knowledge of antiviral responses in chickens. Keywords: chicken TRIM25, subgroup A of avian leukosis virus, antiviral bioactivities, type I interferon response Introduction Tripartite motif 25 (TRIM25) is a tripartite motif (TRIM) protein family member and is characterized by a conserved structural domain at the N terminus, including a *Correspondence: [email protected] † Jin-run Zhou, Jun-hong Liu and Hong-mei Li contributed equally to this work 1 Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Tai’an 271018, China Full list of author information is available at the end of the article
catalytic RING domain, two B-box domains, and a coiledcoil dimerization domain [1, 2]. In addition, TRIM25 has a C-terminal SPRY domain that binds to target proteins, such as the RIG-I receptor [3, 4]. TRIM25 was first reported in 1993 as an oestrogenresponsive finger protein (EFP) that regulates the oestrogen response [5]. Later, it was also found that the protein can mediate RNA virus infection and induce the antiviral response by ubiquitinating intracellular effectors [6, 7]. TRIM25 ha
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