Relationship Between Reactive Oxygen Species and Emodin Production in Aspergillus ochraceus
Content of emodin, enzyme activity of SOD, enzyme activity of CAT and content of ROS were investigated simultaneously in fermentation process of Aspergillus ochraceus LP_318 and LP_276 in order to reveal relationship between Reactive Oxygen Species and em
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Relationship Between Reactive Oxygen Species and Emodin Production in Aspergillus ochraceus Ping Lv
Abstract Content of emodin, enzyme activity of SOD, enzyme activity of CAT and content of ROS were investigated simultaneously in fermentation process of Aspergillus ochraceus LP_318 and LP_276 in order to reveal relationship between Reactive Oxygen Species and emodin production for speculating the mechanism of high-yield emodin from Aspergillus ochraceus LP_318. The result showed that emodin was identified as an antioxidation in vivo and an explosion of ROS which was found from 36 to 84 h in LP_318 stimulated to produce emodin efficiently with deficiency in enzyme activity of SOD in LP_318. Keywords Reactive
Oxygen Species Emodin SOD CAT
288.1 Introduction Emodin or 1,3,8-trihydroxy-6-methyl-anthraquinone (Fig. 288.1) is a naturally occurring pigment found in many plants [8], molds and lichens, exhibiting diverse biological activities including anticancer functions [10, 12, 13] and anti-inflammatory functions [5]. Early studies on its therapeutic benefits were mainly focused on its laxative functions as it is abundant in traditional Chinese medicinal herbs used for laxative formulation, such as rhubarb, the root and rhizome of Rheum palmatum L [15]. The health benefit of emodin has been linked to its involvement in many cellular processes, such as the suppression of tumor-associated angiogenesis through the inhibition of extracellular signal-regulated kinases [9]. There was also a report that emodin can sensitize certain types of breast cancer cells to the treatment of paclitaxel [16]. Its inhibitory effect on tumorogenesis-associated P. Lv (&) Department of Biotechnological and Environmental Engineering, Tianjin Professional College, Tianjin 300410, China e-mail: [email protected]
S. Li et al. (eds.), Frontier and Future Development of Information Technology 2337 in Medicine and Education, Lecture Notes in Electrical Engineering 269, DOI: 10.1007/978-94-007-7618-0_288, Springer Science+Business Media Dordrecht 2014
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P. Lv
Fig. 288.1 Chemical structures of emodin
cell signaling pathways has made emodin an interesting molecular entity for antineoplastic studies and formulations. Recently, we reported our studies on a strain of Aspergillus ochraceus isolated from Chinese potato, which constantly produced emodin which were isolated using Silica gel column chromatography and purified by preparative high performance liquid chromatography [11]. It was very important that the induced mechanism of Emodin from Aspergillus ochraceus was studied for Industrialization production of emdon based on high yield fermentation. At the same time, two polymorphic amplification segments about producing emodin were found by RAPD analysis. One was homologous with XM_002374227.1 and AY585205.1, which indicated the biosynthesis of emodin was related to SOD and oxygen free radicals. (Data unpublished). It was well known that Emodin, a natural anthraquinone polyphenol, was reported to possess promising in vitro anti
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