Relationship between the Levels of mRNA Expression for Protein Phosphatase 1B and Proteins Involved in Cytoskeleton Remo
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Bulletin of Experimental Biology and Medicine, Vol. 169, No. 4, August, 2020 ONCOLOGY
Relationship between the Levels of mRNA Expression for Protein Phosphatase 1B and Proteins Involved in Cytoskeleton Remodeling in Squamous Cell Carcinoma of the Larynx and Hypopharynx
G. V. Kakurina, E. S. Kolegova, E. E. Shashova, V. V. Velikaya, Zh. A. Startseva, O. V. Cheremisina, E. L. Choinzonov, and I. V. Kondakova Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 169, No. 4, pp. 491-494, April, 2020 Original article submitted October 24, 2019 We analyzed the expression of genes encoding proteins involved in cytoskeleton remodeling (RND3, SNAI1, vimentin, cofilin, adenylate cyclase-associated protein 1, ezrin, and profilin) depending on the level of expression of protein phosphatase 1B (PPM1B) mRNA on the example of squamous cell carcinoma of the larynx and hypopharynx. Against the background of a high level of PPM1B expression, a significantly high level of profilin expression was noted. Metastasis correlated with the level of snai1 expression, while relapse after combination treatment was negatively associated with the level of vimentin expression. The obtained new data can reflect molecular peculiarities of the tumor growth in squamous cell carcinoma of the larynx and hypopharynx. Key Words: protein phosphatase 1B; signaling proteins; actin-binding proteins; squamous cell carcinoma of the larynx and hypopharynx; progression Protein phosphorylation/dephosphorylation is a universal regulatory mechanism for various cellular processes (protein synthesis, signal transmission, gene transcription, etc.) under normal conditions. Much recent attention is attracted to the role of these processes in tumor growth [2,3,11,12]. Phosphorylation/dephosphorylation cycles are provided by a large number of protein kinases and protein phosphatases [2]. The latter include protein phosphatase 1B (PPM1B) identified by the proteomic analysis of the blood serum from patients with squamous cell carcinoma of the larynx and hypopharynx (SCCLH) in our previous study. Further studies revealed the dependence of the tissue level of PPM1B on the size of the primary tumor node and the presence of lymphogenous metastases in patients with SCCLH [1]. PPM1B (PP2CB) belongs to the classic serineCancer Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences, Tomsk, Russia. Address for correspondence: [email protected]. G. V. Kakurina
threonine phosphatases and is involved in the various regulatory signaling pathways, including regulation of proliferation (TAK1→NFκB pathway), transcription, and apoptosis (Wnt-signaling pathway). The role of PPM1B in the tumor growth remains little studied and practically not described. Our aim was to study the relationship between the expression of the gene encoding PPM1B and genes of proteins involved in the implementation of cell motility, in particular, mRNA of signal molecules (RND3, SNAI1) and cytoskeleton proteins vimentin, cofilin, adenylate cyclase-
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