Evaluation of gene expression and protein structural modeling involved in persister cell formation in Salmonella Typhimu
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BACTERIAL, FUNGAL AND VIRUS MOLECULAR BIOLOGY - RESEARCH PAPER
Evaluation of gene expression and protein structural modeling involved in persister cell formation in Salmonella Typhimurium Negar Narimisa 1,2
&
Fatemeh Amraei 1,2 & Behrooz Sadeghi Kalani 3,4 & Faramarz Masjedian Jazi 1,2
Received: 20 January 2020 / Accepted: 16 October 2020 # Sociedade Brasileira de Microbiologia 2020
Abstract Persisters are phenotypic variants of the bacterial population that survive against lethal doses of bactericidal antibiotics.These phenotypes are created in numerous bacterial species, including those of clinical significance, such as Salmonella Typhimurium. Since persister cells are associated with the failure of antibiotic treatment and infection recurrence, it is crucial to identify the mechanisms that influence the formation of these cells. The aim of this study is to investigate the persister cell formation and expression analysis as well as to predict the 3D structure of the genes involved in the production of persister cells. The presence of persisters in S. Typhimurium was determined by time dependent killing of different types of bactericidal antibiotics and expression of genes associated with persister cell formation which was assessed five hours after the addition of antibiotics by the qRTPCR. Indeed, the 3D structural model of the proteins studied was predicted by performing several computational methods of retrieved primary protein sequences. The results of the study showed that the S. Typhimurium produced high levels of persister cells in the exposure of bactericidal antibiotics. Furthermore, qRT-PCR resulted in the fact that the expression of related genes was different depending on the type of antibiotic. Overall, this study provides information on the creation of persister cells and the role of different genes in the formation of these cells and structure of proteins involved in the production of persister cells in S. Typhimurium. Keywords Salmonella Typhimurium . qRT-PCR . Persister cell . 3D structure
Introduction Persister cells are defined as part of the bacterial population that survives against lethal doses of antibiotics [1]. These cells, with genetic and antibiotic minimal inhibitory concentration (MIC) values, are similar to antibiotic susceptible cells in the exposure of antibiotics by phenotypic switching which cause inactivation of physiological state, slow growing, and Responsible Editor: Cristiano Gallina Moreira. * Faramarz Masjedian Jazi [email protected] 1
Microbial Biotechnology Research Center, Iran University of Medical Science, Tehran, Iran
2
Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
3
Department of Medical Microbiology, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran
4
Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran
dormancy and can tolerate high concentrations of antibiotics [2]. However, after the antibiotic removal, the persister cells regrow and form a
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